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We narrowed to 1,046 results for: ins;

Showing: 441 - 450 of 1046 results
  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...multiplexing of sgRNAs against unique genomic targets or multiple sgRNAs against the same target to ensure...protein purification and metabolic engineering. If instead you want to build genetic circuits and would benefit...library of parts but don’t need to add tags to your proteins, then the CIDAR Kit is an excellent option (Iverson...system includes a toolkit to express recombinant proteins within the mitochondrial matrix.     Figure...can be repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized...of a Toolkit for the Expression of Heterologous Proteins in Yeast Mitochondria.” BMC Biotechnology, vol...
  2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ... ZIF268 DNA binding domains fused to either the hER or hPR ligand binding domains and the MSN2 transactivation...Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...TRIM-Away is suitable for both short-and long-lived proteins, and it can even distinguish between protein variants... STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment ...plasmids encoding fluorescent reporters and other proteins controlled by GAL4 and ZIF268. To demonstrate ...
  3. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...plasmid is the correct expected size and contains the correct insert Sequence the regions that have been amplified...DNA. As a general rule, try to use an excess of insert compared to the backbone plasmid; a starting point...point could be a molar ratio of 1:2 (plasmid:insert), but this parameter has to be optimized according...transforming the ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different...primers. If not, design primers to sequence your insert. When you get your sequencing results back, you...
  4. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides...our fourth year working with the Rare Genomics Institute by providing plasmid-based tools for rare disease...Addgene to set up assays to see what molecules or proteins are involved in IL-36 release in keratinocytes... valve (an aortic valve that has two leaflets, instead of three) and acute aortic dissections (a tear ...standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization, signalling...Resources Find out more about the Rare Genomics Institute and this year's BeHeard Award! ...
  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...genomic deletions with or without small insertions. But, instead, PEDAR couples this deletion process to...mammalian cells, especially when fused to effector proteins for gene activation or gene editing. The more ... lab adapted the native PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid...it is also capable of coding concurrent small insertions to allow in-frame deletions and introduce epitope...mouse model of tyrosinemia, where a pathogenic insertion within the FAH gene was precisely corrected, restoring...
  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... learned over the years with the hope that our insights can help steer you in the right direction when... in our current setup, there are no explicit instructions on what to do when something fails. While we... through which she could easily transfer her instructions for dealing with the samples to newer lab members... ourselves up for success by giving explicit instructions to lab members through the software if there...collisions you may encounter: vector, sequence, insert, trait, factor, expression, etc.There are also ...biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have...
  7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...As Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working...create an almost limitless number of DNA binding proteins with Cas9 guided by different promoters. The challenge... genetic circuits is getting enough regulatory proteins that don’t interfere with each other. CRISPR is...much easier it is to work with than regulatory proteins. There are still things we haven’t solved that...Nothing too much more sophisticated. There are also proteins, like antibodies. But we are really just using...
  8. Working with Nuclear Receptors

    Type
    Blog Post
    ...controlled by deliberate and compulsory actions instigated by the central nervous system signaling to peripheral...sensors for a diverse set of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are...progesterone, androgens, and estrogens), fat-soluble vitamins A and D, thyroid hormone, fatty acids, oxysterols...composed of a tightly-bound invariant group of proteins, whereas the more loosely bound co-coregulators...explain how more than 300 different coactivator proteins individually contribute to cell regulation in ...acetyltransferase to luciferase, the basic assay remains widely used today.  The sheer abundance of these...
  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...or non-existent PAM requirement, and how these proteins are advantageous in today’s genome editing landscape...the site in question (Kwart et al, 2017). To add insult to injury, the most utilized PAM sequence, that...requirements. Luckily, there are some available.   Cas proteins that don’t utilize NGG   There are plenty of Cas9...same PAM preference of NG was isolated by the Kleinstiver Lab (read more on this lab's other developments...developments below!) (Walton et. al, 2020). These Cas proteins have everything we know and love about Cas9, but...and location.   Nearly PAM-less editing   The Kleinstiver lab sought to address the PAM problem through...
  10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range...tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left and...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...CRISPRi to non-model bacteria including pathogenic strains and environmental isolates. For example, Peters...expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold reduction, with...the relative frequency of each of the 40 library strains was measured after 6 and 12 generations with or...
Showing: 441 - 450 of 1046 results