We narrowed to 991 results for: Des

... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

...restriction enzyme digest (subcloning), including design and experimental procedures....easily move YGOI into a mammalian expression vector. Design (Choosing enzymes) Many DNA analysis tools, including..., but do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...your recipient plasmid as well as a specifically designed test digest later to verify that the insert was... fear. You have other options, such as: Adding desired restriction sites to flank your insert : You can...restriction enzymes that cut within your insert. Adding desired restriction sites to your recipient plasmid : You... orientation of your insert, so you may want to design a diagnostic digest for this purpose. Congratulations...

...novel RNA-protein interactions, Ramanathan et al. designed an RNA bait containing three well conserved but...

...these difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills...

... phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 ...

...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

...to the development and spread of cancer cells. Besides these stromal cell modifications, stromal cells...

...Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater® with ...

...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...

...talking about here? Many of the Fab fragments described above can be used for similar applications and...

...down your own results; are they the same as the description the authors give? Ask yourself if the results...

...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...

...Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol describes how ...This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells...type will be able to grow under the conditions described in this protocol. Conditioned medium (details ... specific conditions used. Each 96-well plate described in this protocol could, in theory, give rise to...protocol was performed at Addgene on the A549 cells described in our sample data , each 96-well plate gave rise...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers... Primers Primer design for LIC is often as simple as using the backbone manufacturer's suggested leader...primer is shown here. Note: Use web-based primer design software to ensure a melting temperature between...nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase protocol), causing the...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they...

...uncomfortable places, from sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct...

...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions...