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  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...timers may be sensitive to changes in oxygen, temperature, and pH, but timer activity can be calibrated...dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where...

  2. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... I needed help with: wouldn’t it be fun to get several of the labs in our program to write short, funny...of content they share and produce and how they interact with their followers. It’s also been a great place...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood... CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning on) ...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...amount of time to perform the search as well as the overall alignment results. The default setting is “Optimize...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...remotely-tethered ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive...Created with BioRender.com.   Important considerations for using optogenetic tools Once you’ve selected...

  9. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    .... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through... cells. Once in the cells, amine reactive dyes interact with free amines in the cytoplasm causing dead...

  13. The 12 Days of CRISPR

    Type
    Blog Post
    ...variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, Addgene gave...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...

  16. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...reagents to a single tube and simply flick the tube several times. This will help ensure ensure equal distribution...
Showing: 881 - 900 of 998 results