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We narrowed to 1,009 results for: Eras

Showing: 881 - 900 of 1009 results
  1. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...and synthetic biology Synthetic biologists have leveraged the power of Golden Gate cloning into a modular...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...
  2. Engaging with science and society at pgEd

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    Blog Post
    ... of society or devoid of social context. This “general public” audience comprises the taxpayers who fund...be affected by the knowledge and applications generated from this research. At both a practical and ethical...
  3. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...
  4. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood... CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning on) ...
  5. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ...timers may be sensitive to changes in oxygen, temperature, and pH, but timer activity can be calibrated...dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where...
  6. PhD Applications After COVID

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    Blog Post
    ...other departments/laboratories? How long is the average PhD in this department? How has the department ...research focuses on Drosophila-parasite pathogen interactions.  ...
  7. Common Injection Routes in Mice

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    Blog Post
    ...purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds very ... that require ingestion, such as experimental therapies or pain relief, straight to the stomach. This ...
  8. Experimenting with New Careers while in Grad School

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    Blog Post
    ... I needed help with: wouldn’t it be fun to get several of the labs in our program to write short, funny...of content they share and produce and how they interact with their followers. It’s also been a great place...
  9. Tips for Using BLAST to Verify Plasmids

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    Blog Post
    ...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...amount of time to perform the search as well as the overall alignment results. The default setting is “Optimize...
  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...
  11. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...remotely-tethered ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive...Created with BioRender.com.   Important considerations for using optogenetic tools Once you’ve selected...
  12. Antibodies 101: Flow Cytometry

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    Blog Post
    .... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through... cells. Once in the cells, amine reactive dyes interact with free amines in the cytoplasm causing dead...
Showing: 881 - 900 of 1009 results