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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,023 results for: NTS

Showing: 881 - 900 of 1023 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    ...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

  2. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally...for sequencing, representative NGS results (read counts or another visualization) that confirm diversity...

  5. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...exposure. However, the limited availability of reagents and equipment and the long turnaround times, have...steps and the Cas protein used. Here, all the components needed for the reaction are mixed in a single...

  8. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...isotype controls are also commonly used in IF experiments. Precisely which controls you perform will depend...spectral properties match your available imaging instruments and the spectral properties do not overlap with...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase ...needs to be taken into account when designing experiments.   {% module_block module "widget_0c4dbd65-f69b...

  10. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...digest appears normal and produces the expected fragments for that plasmid. This phenomenon indicates the...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...that isolates proteins from different cellular compartments such as the mitochondria, cytoplasm and nucleus...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase...

  16. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...you will find this information useful for your experiments. Don’t hesitate to check our lentiviral vector...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...more genes, protein coding sequences, or other elements inserted into it. You will have the option to ...plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is a Bacterial...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant...2018). Real‐time observation of flexible domain movements in CRISPR–Cas9. The EMBO Journal, 37(10), e96941...

  20. CRISPR Guide Update

    Type
    Blog Post
    ...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...
Showing: 881 - 900 of 1023 results