Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,082 results for: ica;

Showing: 881 - 900 of 1082 results

  1. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols...

  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...

  6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ... specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...PI labs and institutions. There are many more technical details that make our new search engine unique...

  8. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...

  9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

  10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna...

  11. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

  12. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...directed evolution, because channelrhodopsins are typically evaluated with low-throughput methods such as ... midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...to your values While going through the co-op application process, my advisor encouraged me to keep my ...

  14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...s outer membrane and allows the components to physically enter the cells so they can go to work. ...Allen Cell Explorer, where you can also find the certificate of analysis provided with each cell line. The...

  15. Lab Automation at Addgene

    Type
    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...using either loxP or lox511 sites, leaving two identical sites on one end of the DNA fragment (Figure 1...recombination event then excises the DNA between the identical loxP or lox511 sites (Figure 1, bottom). Since...presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead of BFP....

  17. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...instances. If your poster material is nearing publication, consider uploading your work to a preprint server...time to meet. Some conferences may also have a dedicated app with a social media component, but if it doesn...other posts on scientific sharing, scientific communication, and scientific networking Learn how to set ...

  18. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...:  my undergrads were different people! More specifically, they had different learning styles. One was...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

  19. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...however, your cost per cloning reaction drops dramatically, as one tube of enzyme should be enough for ...there is no need for restriction digests, gel purifications, or ligations. The general Gateway cloning protocol...Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR ...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ...
Showing: 881 - 900 of 1082 results