...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna...

... the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...directed evolution, because channelrhodopsins are typically evaluated with low-throughput methods such as ... midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light...

...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...

...s outer membrane and allows the components to physically enter the cells so they can go to work. ...Allen Cell Explorer, where you can also find the certificate of analysis provided with each cell line. The...

... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...to your values While going through the co-op application process, my advisor encouraged me to keep my ...

...using either loxP or lox511 sites, leaving two identical sites on one end of the DNA fragment (Figure 1...recombination event then excises the DNA between the identical loxP or lox511 sites (Figure 1, bottom). Since...presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead of BFP....

...however, your cost per cloning reaction drops dramatically, as one tube of enzyme should be enough for ...there is no need for restriction digests, gel purifications, or ligations. The general Gateway cloning protocol...Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR ...

...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ...

...:  my undergrads were different people! More specifically, they had different learning styles. One was...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

...directions. Drosophila are very easy to manipulate genetically and have limited genetic redundancy (meaning,...reference of various fly phenotypes, check out the Classical Genetics Simulator website. One interesting thing... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

...instances. If your poster material is nearing publication, consider uploading your work to a preprint server...time to meet. Some conferences may also have a dedicated app with a social media component, but if it doesn...other posts on scientific sharing, scientific communication, and scientific networking Learn how to set ...

... to synthesize a large volume of information: basically, a scientist’s tool kit. I wrote a review article... was cheating. Eventually I learned that it’s practical because there simply isn’t enough time to read...used both. Curate and present some useful data Typically lists and pictures are the most useful parts of...

...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...is simple -  no restriction, ligation or gel purification steps are required! Multiple fragment cloning...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...

... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...

... use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two ...thus marking cells that have undergone genomic modification. In this system, Cas9 must be supplied on a ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...

... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

... showed that a laughter program significantly decreased immunological markers of stress in adolescents...

...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...