We narrowed to 941 results for: ina

1. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to engineer...

3. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ... number of target DNA molecules present in the original sample with a great deal of precision. This means...

4. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   ...as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your choice...

5. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   .... (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac...

6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   ...consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two...

7. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

8. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   ...energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his...

9. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   ...feedback for improvement. Go to a science or career seminar together and meet to debrief on the topic (career...

10. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ... you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for...

11. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...comfortably engage with you. If you can give a seminar, lead a journal club, or present at lab meetings...

12. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require...

13. All in a Twist: dsRNA

    Type

    Blog Post

    ... allowing the DNA strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle...

14. Antibodies 101: Conjugation

    Type

    Blog Post

    ...Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for...

15. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    ...could meet your needs.    Multiplex experiments Finally, consider whether you will be using other antibodies...

16. What's New in CRISPR - November 2021

    Type

    Blog Post

    ... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...

17. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    ...of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    .... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

19. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board...

20. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety ...