We narrowed to 941 results for: ina
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TypeBlog Post... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks...
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Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to engineer... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post... number of target DNA molecules present in the original sample with a great deal of precision. This means... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your choice... -
Plasmids 101: Blue-white Screening
TypeBlog Post.... (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...feedback for improvement. Go to a science or career seminar together and meet to debrief on the topic (career... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post... you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...comfortably engage with you. If you can give a seminar, lead a journal club, or present at lab meetings... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require... -
All in a Twist: dsRNA
TypeBlog Post... allowing the DNA strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle... -
Antibodies 101: Conjugation
TypeBlog Post...Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...could meet your needs. Multiplex experiments Finally, consider whether you will be using other antibodies... -
What's New in CRISPR - November 2021
TypeBlog Post... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post.... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety ...