Hot Plasmids - May 2022
Type
Blog Post
...specific subcellular localization peptide. By combining three different localization peptides with four...
We narrowed to 985 results for: nin
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OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post... Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1).... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...visualized simultaneously. Complementation systems for examining protein-protein interactions Split luciferases... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...than you – meeting scientists at your level of training is always a good idea. Make contact with other... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including... -
Human Germline Editing Using CRISPR
TypeBlog Post...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes... -
CRISPRainbow and Genome Visualization
TypeBlog Post...CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent... -
All in a Twist: dsRNA
TypeBlog Post...associated with bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...subsequent cell divisions, the fraction of cells containing the particle exponentially decreases over time... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... the major drawbacks of the indirect method, lengthening an often already lengthy process. (And while ... -
Summer SciComm: Preprints
TypeBlog Post...opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles in ... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post.... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post... papers is to practice reading them. There’s a learning curve for the jargon and background in your field... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...there’s a lot of control spatially as to what’s happening at different points within the wood. There are... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...lines and these cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation... -
Lab Automation at Addgene
TypeBlog Post...receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available on our...