...   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." ...

... your DNA sequence Finally, when you do see a miscalled peak, don’t be shy. Feel free to edit it. Most...

...disorders are frequently overshadowed by other medical conditions, so these topics have become a cornerstone...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...

...about how my ideas would be translated through Zoom calls and Google docs. I pondered on the ways that I wouldn't...

...Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year, she’ll mix...

...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...

...the carats in the datatable headers to sort alphabetically or in ascending or descending order, in the...

...accomplishments, research/professional goals and feedback. Calendar: Month-by-month look at the next year, including...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...transfer plasmid sequences into the host genome. Typically it is the sequences between and including the ...whereas standard retroviruses can only infect mitotically active cell types. This means that lentiviruses...Lentiviral transfer plasmids are not designed specifically for transient transfections. Therefore, there...and cloned into the lentiviral vector. This is basically shuffling restriction sites between vectors until... entry vector at a region flanked by sequences (called attP1 and attP2) that allow the insert to recombine...

...benefited from the business rigor and efficiencies typically adopted by for-profit companies, while making ...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...

...exploration in this area will accelerate the launch of clinical applications. During the panel discussion, Dr...

...even changes the DNA sequence through a mechanism called depurination. In depurination, the hydrolysis of...

...easier to ship back. Be that person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, ...

...Runne-Janczy’s first day at eScience Labs was not your typical first day. “I walked in and they asked me, 'How...

... when she signed up for a 10 week class at Iowa called “Teaching Your Research.” This course has participants...

...sessions and reinforced our newfound knowledge with practical understanding of how to apply it. The personal...

...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...