We narrowed to 994 results for: Des
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TypeBlog Post... Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale in screens...
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CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post.../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy... -
Engaging with science and society at pgEd
TypeBlog Post...uncomfortable places, from sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning capacity, decreased ... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...specific protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of ... -
Lab Automation at Addgene
TypeBlog Post...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find... -
Viral Vectors 101: Virus Safety
TypeBlog Post...that are built into how something functions, were designed for this purpose. One such control is dividing... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post... and to work in a variety of applications. The desired specificity of your secondary antibody depends ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...v) HCl Adjust to pH 2.0–2.2 For the mildest stripping solution, omit the Tween 20. Once your... -
6 Tips for Grant Writing
TypeBlog Post...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps required... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...communication and learning to work with people despite the distance improved my verbal and written communication... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...Derek Jacoby from Makerspace Victoria, CA Over the decades science has become increasingly restricted to academic... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...familiar with the minute details of your work. Describe the problem by stating the facts and explain how... -
Ligation Independent Cloning
TypeProtocol...experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers... Primers Primer design for LIC is often as simple as using the backbone manufacturer's suggested leader...primer is shown here. Note: Use web-based primer design software to ensure a melting temperature between...nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase protocol), causing the...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they... -
Transferable Skills: Negotiation
TypeBlog Post...specific details about the individuals, each example provides a scenario where both people get something valuable... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional... -
10 Basic tips for mammalian cell culture
TypeBlog Post...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers...