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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 974 results for: LAT

Showing: 901 - 920 of 974 results

  6. DNA Purification Without a Kit

    Type
    Blog Post
    ...tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run ...

  9. Lab to Office Culture Shock

    Type
    Blog Post
    ... yourself a Scientist. You work in a lab. You formulate hypotheses and conduct experiments. You transfer...

  15. Typing CRISPR Systems

    Type
    Blog Post
    .... During this time, scientists discovered many related immune mechanisms that could target foreign DNA...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... an automated ELISA plate washer. Seal the plate. Place the plate on a microplate shaker set at 400 rpm...use an automated ELISA plate washer. Seal the plate Place the plate on a microplate shaker set at 400 rpm... the plate with a plate seal and wrap in foil. Incubate plate at room temperature on a microplate shaker...each row of a 96-well ELISA microplate. Cover the plate with a 96-well plate seal and incubate at 37 °C... to each well. Cover the plate with a plate seal and incubate on a microplate shaker set to 400 rpm for...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...overnight. Plate 2.5 mL of the transformed cells on each of the eight bioassay plates (two plates per tube...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate plates at 30 ℃ overnight... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight... are not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12...LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ... forget to factor this into your calculations later (see calculation example below). Seed 300,000 cells...well plate sealer by gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto... Foil Heat Seal on the PCR plate with the red line facing up. If the plate sealer is not at temperature...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ...transfer the plate to the Droplet Reader. Open the QuantaSoft software to set up a new plate layout. Designate... a CSV file which will be used to calculate the titer. Calculations In the experimental setup above, the...dilution into 6-well plate 2.5 25 5 50 10 100 20 200 40 400 80 800 160 1600 To calculate the titers, first...

  20. AAV ddPCR Titration

    Type
    Protocol
    ...02-FCP-C ddPCR 96-well PCR plates, Bio-Rad, 12001925 48-well dilution plate, Bio-Rad, MLL4801 Pierceable... Foil Heat Seal on the PCR plate with the red line facing up. If the plate sealer is not at temperature...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ...transfer the plate to the Droplet Reader. Open the QuantaSoft software to set up a new plate layout. Designate...compliance with your institution’s biosafety regulations. Equipment Class II, Type A2 Biological Safety...Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1–10 µL single channel pipette...reagents and the NTC with virus. Pre-warm the 96-well plate sealer by gently touching the screen. Prepare the...
Showing: 901 - 920 of 974 results