We narrowed to 1,076 results for: TOR
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TypeProtocol...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of... up your work area and microscope after use and store the microscope appropriately....
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CRISPR 101: Validating Your Genome Edit
TypeBlog Post...performing a single base pair edit with a prime editor. What next? When generating a knock-in mutation... -
Pouring LB Agar Plates
TypeProtocol...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She... -
Protocol - How to Streak a Plate
TypeProtocol...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ... -
Water Bath Protocol
TypeProtocol... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room... -
Deep Dive: qPCR
TypeBlog Post...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type drop down menu and select...calculate this for you as follows: In the Chart Editor , select the Customize tab. Select the Series drop... -
Western Blot
TypeProtocol...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place... -
DNA Quantification
TypeProtocol... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many... -
Protocol - How to Perform Sequence Analysis
TypeProtocol...important for your experiments. There are a number of factors to consider when selecting sequencing primers, ... -
Weighing Reagents Protocol
TypeProtocol... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect... -
Gibson Assembly Protocol
TypeProtocol...especially useful for introducing promoters, terminators, and other short sequences into the assembly ... -
Protocol - How to Run an Agarose Gel
TypeProtocol... After all of the sample is unloaded, push the pipettor to the second stop and carefully raise the pipette... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...curve or dilute the unknown sample (don’t forget to factor in that dilution when calculating the original ... -
Immunology Research Plasmids and Resources
TypeCollection...receptor C MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RXFP1 relaxin/insulin-like family peptide receptor...IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated...cytokine receptor-like factor 1 CISS, CISS1, CLF, CLF-1, NR6 CRLF2 cytokine receptor-like factor 2 CRL2,...cytokine receptor-like factor 3 CREME9, CYTOR4, FRWS, MGC20661 CSF1 colony stimulating factor 1 (macrophage...growth factor receptor 3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 FGFR4 fibroblast growth factor receptor 4 CD334...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, ...CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor MGC1774 CORT cortistatin... -
Fluorescent Protein Guide: Biosensors
TypeCollection...and green GPCR-based NE indicators Sensitive multicolor indicators for monitoring norepinephrine in vivo...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...calcium indicators for optical imaging (GECOs), including blue, green, red, and ratiometric indicators An ...encoded calcium indicators. J Biol Chem. 2019 Mar 15;294(11):3934-3946. Katalin Torok Calcium Red Calcium...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging...Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators visualize spatiotemporal... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry...