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...While the various AAV serotypes can be used to achieve different patterns of expression, researchers often...

...GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems provide efficient...

... Susanna Bachle...

...will be collected, what the hypotheses are, and how each hypothesis will be tested with a specific statistical...

...proteins has many useful genome engineering enzymes, each with their own unique features and quirks. Here,...

...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

...want to meet or that you think might be helpful to each other. This is a quick way to do an easy favor for...

...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

...on the extracellular matrix and fibroblasts. With each cell mechanism discovered in microenvironments, ...

...arbitrary DNA sequences and have been shown to achieve ~100 fold repression in E. coli. In addition, TALEs...

...work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but it’s toxic to tissues...

...design. We all want tools that expand what we can achieve, but are simple to pick up and work well the first...

... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

... making fluorescent protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent...

...regeneration of ATP from ADP is creatine. In order to reach the brain, creatine needs to be transported from...

...Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools means ...

...and Western Europe have taken three strategic approaches to gender equality over the past several decades...

...variety of restriction enzyme-based cloning methods. Each method has its own pluses and minuses, but Golden...

...double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated, resulting...

...exposure times. To test the antibody’s reproducibility, each antibody is independently screened twice, confirming...