We narrowed to 949 results for: ache

1. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   ...and Western Europe have taken three strategic approaches to gender equality over the past several decades...

2. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   ...variety of restriction enzyme-based cloning methods. Each method has its own pluses and minuses, but Golden...

3. Plasmids 101: Cre-lox

   Type

   Blog Post

   ...double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated, resulting...

4. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   ...example, in our hands we find that a double harvest approach at 48 and 72h produces high titer virus for transfer...

5. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ..., Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form Addgene to ...

6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   ...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...

7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   ...has helped me realize that is not true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming...

8. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   ...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...

9. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   ...dependent on the ability of agonists to successfully reach target cells while resulting in minimal off target...

10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...COVID-19 related plasmids. These plasmids have reached labs in over 75 countries. Figure 1: SARS-CoV...

11. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...that encode those amino acids (Tran et al., 2016). Each antibody would then be tested for antigen binding...

12. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ...pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with...

13. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    ...exposure times. To test the antibody’s reproducibility, each antibody is independently screened twice, confirming...

14. 6 Tips for Grant Writing

    Type

    Blog Post

    ...technologies to solve a problem can allow you to approach that problem in a novel way. Finally, engineering...

15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ...choose essentially any background, but two common approaches are either a solid color, or a “themed” background...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ...ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used to non-invasively...

17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...LbCpf1 (blue). Mismatch positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016...

19. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...funded on a membership model - everyone contributes each month - with only occasional supplementation with...

20. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...background without an introduction. If someone approaches your poster, introduce yourself and ask about...