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Showing: 901 - 920 of 1082 results
  1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post
    ...directions. Drosophila are very easy to manipulate genetically and have limited genetic redundancy (meaning,...reference of various fly phenotypes, check out the Classical Genetics Simulator website. One interesting thing... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...
  2. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...
  3. How to Write a Scientific Review Article

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    Blog Post
    ... to synthesize a large volume of information: basically, a scientist’s tool kit. I wrote a review article... was cheating. Eventually I learned that it’s practical because there simply isn’t enough time to read...used both. Curate and present some useful data Typically lists and pictures are the most useful parts of...
  4. Plasmids 101: Gateway Cloning

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    Blog Post
    ...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...is simple -  no restriction, ligation or gel purification steps are required! Multiple fragment cloning...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...
  5. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ... use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two ...thus marking cells that have undergone genomic modification. In this system, Cas9 must be supplied on a ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...
  6. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...
  7. Addgene at SfN23!

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    Blog Post
    ...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...
  8. The Codon Guide to Synthetic Biology

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    Blog Post
    ...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...
  9. Find and Share AAV Data with Addgene's New AAV Data Hub

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    Blog Post
    ...open platform for scientists using AAV to share practical information about their experiences. It will contain...important details that you won’t find in the final publication and we have already collected over 100 entries...
  10. Twelve Amazing Guest Blog Contributors!

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    Blog Post
    ...Rob Hurt, whose expertise and suggestions were critical to the development and writing of two blog posts...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...
  11. 8 Great Depositor Research Stories

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    Blog Post
    ...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...
  12. New Educational Resource: The Addgene Videos Page

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    Blog Post
    ...Videos page! Videos are a powerful tool for communicating scientific knowledge. On this new page, we have...  Our first video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our...
  13. Antibody Data Repositories and Search Engines

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    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...
  14. CRISPR Cheat Sheet

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    Blog Post
    ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...
  15. Countdown to Halloween @Addgene

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    Blog Post
    ...plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid...creative hijinks last year. They showed us their dedication to great customer support by dressing up as our...
  16. New Viral Vectors - Spring 2025

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    Blog Post
    ...skipping the preparation steps of production, purification, and titering. Our newest viral vector service...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...
  17. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...serotype, species, or promoter) can save you significant time and money. For instance, this report shows...tropism* (min. 300 dpi, PNG format) At least two replicates of your experiment* Functional data, if the report...
Showing: 901 - 920 of 1082 results