.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could...

...PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane...

...Stability: This parameter is important if you are planning to express the FP in acidic environments (e.g....

... together to form the RNP. Detailed protocols outlining these steps have been made publicly available ...

... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...

...necessary to isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed...

...read the paper on bioRxiv.   Using the ICE tool Learning how to use the ICE software tool is quick and ...

...comprehensive lab experience. With this valuable training behind you (and largely only that is necessary...

... genOway, we are able to distribute materials containing FLEx technology. Read the genOway press release...

...biomedical research in the U.S. and postdoctoral training has not been demonstrated to be required for the...

... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive...

...required to present research plans that went beyond obtaining “omic” data and had to include clear plans to ...

...possible to import Word documents into RSpace, maintaining the original formatting. In this way data is ...

...demonstrated that GO is an effective reporter for adenine base editors, that make A-to-G edits in the genome...

... off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside...

...diagnostic tool. A pool of DNA or RNA nucleotides containing a sequence of interest (red) is amplified using...

...reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12 (2013): 1219. PubMed...

...sufficient for greater than 50 percent of RSM-containing cells in a population to correctly record each...

...others. Each test has different likelihoods of returning false positives/negatives, but they all generally...

...preparations that confer antibiotic resistance. Determining the titer of your lentiviral preparation allows...and selection markers. Note this assay requires staining resistant colonies with crystal violet solution...transduce cells Day 2: Replace media with fresh media containing selection reagent Days 3–14: Change media as ...Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative ...alternative stable glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore...-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of...Scientific CC0456-500ML 0.22 µm luer-lock filter, Corning 431229 10 mL luer-lock syringe, BD BD300912 0.45...