Lab Automation at Addgene
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Blog Post
...receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene...
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Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...single-molecule tracking in live cells. Controls When designing experiments, it’s important to include proper ... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...mixture is added to the wells. Any free antibody (meaning it hasn’t bound to your protein of interest) will... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...analyzes publications and figures using machine learning to extract important features such as influential... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...departments across the university. Collective bargaining may provide a better deal for students in areas... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their email address... -
Of Myc and Men
TypeBlog Post...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance... -
PhD Applications After COVID
TypeBlog Post...occurs in the few months leading up to applications opening. With virtual options, you can narrow down your... -
Plasmids 101: Degron Tags
TypeBlog Post...tags we will discuss here are all conditional, meaning that they only facilitate degradation of their ... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...Knockouts with CRISPR through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...Stability: This parameter is important if you are planning to express the FP in acidic environments (e.g.... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... together to form the RNP. Detailed protocols outlining these steps have been made publicly available ... -
Plasmids 101: Protein tags
TypeBlog Post... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...necessary to isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... genOway, we are able to distribute materials containing FLEx technology. Read the genOway press release... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...diagnostic tool. A pool of DNA or RNA nucleotides containing a sequence of interest (red) is amplified using... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...required to present research plans that went beyond obtaining “omic” data and had to include clear plans to ...