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We narrowed to 960 results for: App;

Showing: 461 - 470 of 960 results

  1. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the 70,000 or more...community will find these reagents to be useful. Happy screening! Many thanks to our guest blogger, John...

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly creepy science...defective exoskeleton formations. These genes were appropriately named as spook, spookier, phantom, disembodied... grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B: Biological...

  3. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...and fusions represented in this collection, approximately 80% (over 800) of them had not yet been functionally... viability compared to a wild-type control. Approximately ~22% of the mutations, however, significantly...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... size and frequency [3]. There are several applications in which the production of deletions by paired... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...were willing and able to listen to Addgene’s new approach of working with the industry-based scientific ...reduced to days and weeks. As of June 2020, we are happy to have the support of the following organizations...project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...opportunities.  Scientists must do science to be happy so the beginnings of solutions can’t come too soon...

  8. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...expression level. Labs have been trying different approaches to improve CTC capture, but I hope that our antibody...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...
Showing: 461 - 470 of 960 results