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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 461 - 470 of 1046 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... students during an interview. When they were instructed to use the three-ring binder as a starting point...people to agree with the most prominent idea, and instead building an environment where everyone has their... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...

  2. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...terminator. Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning videos made by ...according to their concentration, size, and the desired insert-to-vector ratio using the equation in Figure 3....plasmid volume for MoClo experiments. The term ‘insert’ refers to plasmids carrying each part, ‘vector... the ‘amount of vector’ and the ‘molar ratio of insert to vector’ can be defined by you.   Save time...each transformation and/or use different E. coli strains with higher transformation efficiency. If you’re...We strongly encourage you to sequence the entire insert region of your final plasmid or those you are selecting...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...origin of replication (ori), where DNA replication begins, so a bacterial plasmid needs a compatible ori ... divide. In transient transfection, the vector remains episomal, meaning it stays separate from the genome...synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides work...options doesn’t end there. Make sure you read the instructions for whatever antibiotic you choose to ensure...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...researcher may choose not impose selection on cells and instead to use a vector with a reporter, such as a fluorescent...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...expression while proteins needed for later experiments (cellular tracking for instance) can be controlled...Tamoxifen is added. Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to ...fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in ...In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling, researchers...is fascinated by the question of how genes and proteins coordinate billions of neurons to form the functional...

  5. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...pharmaceutical companies, biotech start-ups, health insurance companies, and governments on the most effective...physicians and patients, shaping discussions with insurance companies about the pricing and coverage of drugs...situations might be especially challenging. For instance, the client might need to get a large amount of...occasions, clients can be excessively demanding. For instance, a client may offer a lot of pointed feedback ...understanding of the molecular behaviors of chemicals and proteins to learn how a particular drug or medical device...therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how our clients’ ...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...unstable in vivo due to poor folding and thermal instability at 37°C (Ouellet et al., 2016) and requires a...riboswitch, metabolite binding to the riboswitch instead activates Spinach fluorescence. In the search for...identified the nucleotides that contribute to the instability of Spinach, and were able to develop an aptamer...Spinach, a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI... cell imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed... Aliyah Weinstein...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ..., R., Weinstock, G., Metzker, M. et al. Genome sequence of the Brown Norway rat yields insights into mammalian...organism in microbiology and biochemistry. Certain strains have been specifically developed to thrive in laboratory...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...amphibians in science with early research providing insight into some of the most significant and fundamental...), 565–571. https://doi.org/10.1159/000323761 Modlinska, K., & Pisula, W. (2020). The Norway rat, from...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic module to activate or recruit...such a strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development (Aljiboury...(Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but ...

  9. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...reporter constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted...should be resistant to Cas9 cleavage. If a plasmid contains the template, site-directed mutagenesis can be...which is dam+). Methylation deficient bacterial strains can be identified by the dam13(-) mutation – you...you’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...functional regions of the plasmid including the insert. This is essential since mutations are potentially...PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ...whether you are perusing classic papers or are brainstorming new technological innovations. Let’s take a ...blotting to screen founder transgenic animals for the inserted transgene because Southern blots have a lower ...digests. A researcher can test the orientation of an insert in only a few hours at low cost with restriction...do not typically rely on Sanger sequencing but instead use methods like pyrosequencing that are parallelized...techniques we use now may soon become obsolete. For instance, the popular genome-engineering tool, CRISPR, ...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...
Showing: 461 - 470 of 1046 results