We narrowed to 1,011 results for: RING
-
TypeBlog Post...proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal, neuroscientists have...
-
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB, as well as engineered... -
Plan Your Experiment
TypeGuide... the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit....gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...the gRNA for CRISPR activity. Because you are delivering the mRNA directly to the cells, there is only...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831... -
Antibodies 101: Flow Compensation
TypeBlog Post... you can always change your compensation values during the analysis portion of your experiment. So, as... -
Plasmids 101: Degron Tags
TypeBlog Post...kDa). This system also requires less genetic engineering than some of the other systems reviewed here.... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...2021. After validating this method through monitoring GFP degradation, this protein compartment was... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...that the fusion protein localizes properly by comparing the signal from the fusion itself to the wild-... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...if the plasmid will be within my lab's budget? Ordering: Check the Price of course! The price of some ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...there are many factors that need to be considered during the planning process. For CRISPR experiments, the... -
Genome-wide Screening Using CRISPR
TypeBlog Post...Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...cross the blood-brain barrier to reach the brain. Delivering the CREATE AAV library via IV screens for capsids... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post... Mary Gearing... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...tubes help to guarantee that no samples get lost during their journey. Take the time to track your samples... -
Plasmids 101: Protein tags
TypeBlog Post...FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...pseudotyping and is not HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral...