We narrowed to 970 results for: Abo;

1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ... to devise an experiment that can tell you more about its function.  Resources and references Resources...

2. Hot Plasmids and Viral Preps - January 2021

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   ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...

3. Viral Vectors 101: Virus Safety

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   ...completely inert, but others can cause diseases. Nervous about handling viruses? That’s okay! In this article we...

4. Antibodies 101: Choosing the Right Isotype

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   ...In a direct approach, that is simple - but what about when you need to use an indirect (primary and secondary...

5. Tips for Titering Your Lentiviral Preps

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   ...drawback of this approach is that it can be quite labor intensive. Target cells are transduced with serial...

6. Hot Plasmids: Winter 2025

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   ...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

7. Viral Vectors 101: Producing Your rAAV

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   ...dehydration (Howard & Harvey, 2017). You can learn more about common AAV applications by reading our Viral Vectors...

8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   ... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

9. Twenty Years of Addgene Sharing: CRISPR

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   ...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

10. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ... Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid ...

11. Hot Plasmids and Viral Preps - May 2021

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    ...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

12. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch Up On Your...

13. Viral Vectors 101: Voltage Indicators

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    ...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

14. Light Sheet Fluorescence Microscopy

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    ...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

15. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

16. Antibodies 101: Producing Recombinant Antibodies

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    ...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

18. Negative Can Be Positive: Open AAV Data with Addgene

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    ...asked why they don’t share, many express concern about sharing negative data they cannot explain, perhaps...

19. An Introduction to Adenovirus

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    ...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

20. Getting to Know Expansion Microscopy

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    ...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....