We narrowed to 954 results for: App;
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TypeBlog Post...refining the process down to a place I could be happy with it. Cycle 5 - The Future! Figure 5: ...
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An Integrin Antibody Toolkit from IPI
TypeBlog Post... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...many of the people who work in them are incredibly happy with their jobs. One of the Saturday Hack Day projects... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...common lab strain and determine whether it is appropriate for propogating your plasmid or carrying out ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...international mentor creates a closer and more approachable relationship for the mentee, where (s)he feels... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...under a fluorescent microscope, the mycoplasma DNA appears in the form of small clusters, apart from the cellular... -
Protocol - How to Create a Bacterial Glycerol Stock
TypeProtocol... plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions, including copy... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol...requirements. Conclusion Although simple, following appropriate PPE protocol goes a long way. It is the easiest... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated... -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol...years to come. Design Briefly, we will design overlapping oligos that once annealed can be cloned directly... -
Fluorescence Titering Assay
TypeProtocol... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered... -
General Transfection
TypeProtocol...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different... -
Transfection for Recombinant Antibodies
TypeProtocol...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...amplified. When these reagents are combined in an appropriate buffer, a series of heating (denaturing) and ... -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocol...~3.3 difference Ct for a 10-fold dilution is appropriate). Quality of duplicates: Exclude duplicates from... -
Plasmid Cloning by PCR (with Protocols)
TypeProtocol...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with a plate seal and ... -
AAV Production in HEK293 Cells
TypeProtocol...incubator for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily... -
Antibody Guide
TypeGuide...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods... -
CRISPR Guide
TypeGuide... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...