We narrowed to 965 results for: App;

1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

2. DNA Purification Without a Kit

   Type

   Blog Post

   ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

3. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

4. Plasmids 101: Protein tags

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   Blog Post

   ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

5. Save Time with Transient Plant Leaf Transformations

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   Blog Post

   ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   Blog Post

   ...Often times, a gene gets its name based on what happens to an organism when a gene/protein is depleted ...

7. CRISPR-mediated Plant Base Editors

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   Blog Post

   ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   ...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

9. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   ...carefully regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients...

10. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    ...like viral preps and libraries were kept at the appropriate temperature for the duration of their journey...

11. An Integrin Antibody Toolkit from IPI

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    Blog Post

    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

12. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ...refining the process down to a place I could be happy with it. Cycle 5 - The Future! Figure 5: ...

13. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...many of the people who work in them are incredibly happy with their jobs. One of the Saturday Hack Day projects...

14. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...common lab strain and determine whether it is appropriate for propogating your plasmid or carrying out ...

15. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...international mentor creates a closer and more approachable relationship for the mentee, where (s)he feels...

16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...under a fluorescent microscope, the mycoplasma DNA appears in the form of small clusters, apart from the cellular...

17. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

    ... plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions, including copy...

18. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type

    Protocol

    ...requirements. Conclusion Although simple, following appropriate PPE protocol goes a long way. It is the easiest...

19. Protocol - Over-Agar Antibiotic Plating

    Type

    Protocol

    ...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...

20. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

    ...years to come. Design Briefly, we will design overlapping oligos that once annealed can be cloned directly...