We narrowed to 1,015 results for: LEA

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   Blog Post

   ...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

3. Troubleshooting Your Plasmid Cloning Experiment

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   ...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

5. Design Tips for Prime Editing

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   ...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...

6. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   Blog Post

   ... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

7. How to Prepare for an Industry Interview

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   Blog Post

   ... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

9. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   Blog Post

   ...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ...

10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

11. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,...

12. Of Myc and Men

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    Blog Post

    ...science happens in a vacuum!  Munro and Pelham learned of these new antibodies and identified Myc1-9E10...

13. Hot Plasmids: Winter 2025

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    Blog Post

    ... your research! If they do (or if they don't!), please consider sharing your experience with other researchers...

14. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...

15. Common Injection Routes in Mice

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    Blog Post

    ...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

16. Production of Virus in Insect Versus Mammalian Cells

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    ... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

17. Antibodies 101: Flow Compensation

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    Blog Post

    ...compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells...

18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ...the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find...

19. Polymerase Chain Reaction Overview and Applications

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    Blog Post

    ...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...

20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...