We narrowed to 1,010 results for: PLE

1. Bringing Sustainable Practices to the Lab: Recycling

   Type

   Blog Post

   ...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

2. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   Blog Post

   ...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   ...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

5. CASTing Off for New Shores in Human Genome Editing

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   ...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   ...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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   ...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

8. Some Like it Hot: Thermostable GeoCas9

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   ...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

9. Plasmids 101: Antibiotic Resistance Genes

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   ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

10. Antibodies 101: Epitope Tags

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    Blog Post

    ... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

11. Meet the New Addgene Board Members!

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    Blog Post

    ...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

12. Fluorescent Biosensors for Measuring Autophagic Flux

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    ...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

13. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

14. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

15. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

16. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post

    ...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...

17. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced...

18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...might make it difficult to use NgAgo to disrupt multiple targets. Are you using NgAgo? Let us know how ...

19. K. phaffii: Rising to the Occasion

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    Blog Post

    ...1990s when they abandoned the system. Since then, multiple biotech and pharmaceutical companies (along with...

20. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    ...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...