We narrowed to 949 results for: ache

1. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ...glycol (PEG). We add 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration of 8%...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   ...heterozygote, and the mutation won’t drift away with each passing generation.  Another feature of balancer...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   ...homology arm repair templates containing the tag for each insertion. Also, it remains challenging to screen...

4. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ...et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...two types of languages: one to communicate amongst each other, like this conversation we are having now ...

6. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...

7. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ...previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this example...

8. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   ... in bacterial, yeast, plant, or mammalian cells. Each of these systems has their advantages and disadvantages...

9. Lentiviral Vector Uses and Overview

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   Blog Post

   ...treatment of a molecular disorder: gene therapy approaches to sickle cell disease. Hoban MD, Orkin SH, Bauer...

10. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...SIN is shorthand for self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the...

11. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    ...you show respect for your own career and it will teach them to respect their own responsibilities. Another...

12. Pouring LB Agar Plates

    Type

    Protocol

    ...distrubtion of agar over the bottom of the plate. Cap each plate after pouring and stack as you pour. If your...

13. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...

14. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ... are 4 bases (A, T, C, and G) and 3 positions in each codon. There are therefore 64 possible codons but...

15. Hot Plasmids Spring 2024

    Type

    Blog Post

    ...Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi...

16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    ...is in stark contrast to Cas9, which requires that each DNA target have high sequence identity to the spacer...

17. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    ...rotating antibiotic treatments, so that exposure to each antibiotic is minimized, while the infection is ...

18. Five Popular Model Organisms

    Type

    Blog Post

    ...al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming...

19. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ...ligand-binding pocket, their sheer size impeding ligand attachment. The five other IPI rAbs bind RGD-binding integrins...

20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ... resulting in cells that use one or the other on each target site.   Figure 3: Optimal cut site...