Addgene

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Plasmids 101: Shuttle Vectors

Type

Blog Post

...for simple transfection experiments. Reporters Finally, in some cases a researcher may choose not impose...
Transferable Skills Guide: Career Planning Resources

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Blog Post

...you don’t get the specific position he or she originally called about, you can keep in touch and reach...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...likely different from what I would have done. And finally the stakeholder accepts the new feature as done...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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Blog Post

... cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ. While other groups have focused...
Quick Guide to Near-Infrared Fluorescent Proteins

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Blog Post

...normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found...
Antibodies 101: Normalization and Loading Controls for Western Blots

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Blog Post

...or membrane (depending on your approach) as your final blot. Quantifying the entire lane of protein provides...
Plasmids 101: Biotinylation

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Blog Post

...PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of Proteins....
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...t suit my collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized...

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