We narrowed to 1,055 results for: ins;

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity... constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting ...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...

...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 ...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...#68307) ) to generate the phosphosites with serine instead of phosphoserine (i.e., a “phosphorylatable” phosphosite...

...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

...Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent...Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...more about the many applications of fluorescent proteins and to find plasmids for your next experiment....collection of genes and markers tagged with fluorescent proteins as well as hundreds of empty backbones. FRET Find...subcellular structures with well-characterized marker proteins. In Vivo Imaging Image with far-red or infrared...open-source, community-editable database for fluorescent proteins created by Talley Lambert. Nikon MicroscopyU (...

...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...is important. However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end...

...with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit Plasmids containing modular inserts that span...reaction Bunz Return to top Adenoviral Strains Browse bacterial strains for adenoviral vector production. ...plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production in Addgene's repository...Shuttle Plasmids Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery... Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...pUMVC Contains MoMLV gag and pol . Use with an envelope plasmid. Bob Weinberg 12371 pCL-Eco Contains MoMLV...includes a reverse transcriptase and an integrase to insert the viral genome into a host cell. Scientists can...packaging genes Envelope plasmid — contains packaging gene env This collection highlights popular gamma-...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672 MSCV-IRES-GFP MSCV...pol , and env . Inder Verma 35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top...

...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...responsible for delivering proteins to the extracellular environment. Proteins are first translated at the...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...assembled a collection of popular fluorescent fusion proteins that are targeted to various organelles or subcellular... (find plasmids for cloning in our Fluorescent Proteins Empty Backbones Collection ). Then, by examining...p63 mTurquoise2 Dorus Gadella 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...F-tractin-EGFP Actin filaments F-tractin EGFP Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell...

...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase...fluorescent proteins. Plasmid...Fluorescence microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or...unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in live animals...background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome photoreceptors...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging...Chemogenetics Fluorescent Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide...

...Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden...environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain...terminators, repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab...Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning ...Link opens in a new window) — Joint Bio-Energy Institute Synberc (Link opens in a new window) — Synthetic...

...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along with key...create fusion proteins with your gene of interest. Plasmid... Plasmid Collections Fluorescent Proteins Empty Backbones...Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared...are prone to dimerization at high concentration. Proteins that are derived from GFP and contain the A206K...Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish/Xenopus/Worm...Expression Return to top Large Stokes Shift These proteins have a large gap between their excitation and ...

...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal D omain...or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...Additional Resources (Link opens in a new window) BrainSpan Atlas of the Developing Human Brain - includes... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA...sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve...Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506 pOpen-Eco31I Eco31I... Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource..., and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's disease research. This collection is part of ...search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...to amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked...