We narrowed to 1,011 results for: RING
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TypeBlog Post... Krüppel associated box (KRAB) is a naturally occurring transcriptional repressor domain which is frequently...
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Bioinformatics at Addgene
TypeBlog Post... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...Central PMCID: PMC5911151. Huang, Qin, et al. "Delivering genes across the blood-brain barrier: LY6A, a... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...Fellow at University of Rochester Medical Center. During development, complex genetic programs specify and... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...non-functional hedgehog exhibit abnormal segmentation during embryogenesis. Typically, regularly spaced denticles... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...activation, only in this case ligand binding would bring the relevant ends together rather than separate ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...typically use a competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing the costs... -
Plasmids 101: Gateway Cloning
TypeBlog Post..., and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB protein ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...of mok results in translation of Mok and Hok, triggering cell death. However, the sok antisense-RNA binds... -
Cloning
TypeGuide...Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson ...are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...Because of the relatively long stretches of base pairing in the annealed product, ligation is rendered unnecessary... -
Five Popular Model Organisms
TypeBlog Post...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post... Mary Gearing... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post... Mary Gearing... -
Adenovirus Guide
TypeGuide...elements into the genome, often altering gene activity. Oncolytic Referring to the destruction of cancer ...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected... Adenoviral vectors are excellent vectors for delivering target antigens to mammalian hosts and have been... -
Plasmids 101: Restriction Cloning
TypeBlog Post...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...function. If the idea of synthesizing your own ligands brings up bad memories of undergraduate organic chemistry... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...validating edits. Mismatch cleavage assays rely on pairing between the edited strand and wild-type strand ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...in our hands). Take-home message In our hands, delivering Cas9 protein in complex with custom synthetic... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post..., it is much easier to genetically modify cells during the reprogramming process as they are propagated...