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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,085 results for: ANT

Showing: 941 - 960 of 1085 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus...

  4. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...

  7. DNA Quantification

    Type
    Protocol
    ... Protocols DNA Quantification DNA Quantification You may also like... Addgene’s DNA Quantification Protocol. Protocols... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

  9. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...adapted for any antibiotic through the use of a selection curve to determine optimal antibiotic concentration...Learn how to add antibiotic to a plate after it's already been poured and solidified. Protocols...Streaking Bacteria on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates...This protocol describes methodology for plating antibiotic over-agar for the selection of transformed E....E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate and... does not need to prepare separate batches of antibiotic-containing agar. This protocol will focus specifically...the protocol video below to learn how to spread antibiotic over-agar. Equipment Pipette tips for both pipetting...

  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific...Collection Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs and When to Use Them...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide Introduction This protocol...Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody incubation...primary antibody and an isotype control antibody to the desired concentrations in the antibody dilution... with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary antibody for 2 ... a purified antigen to demonstrate the expected antibody dose response. Protocols...

  11. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia...

  12. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

  13. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...all published videos in our Quick Tips playlist:  Want your quick tips even quicker? We’ll be sharing our...

  15. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...sweaters, Flannel Fridays, Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout...

  17. CRISPR Guide Update

    Type
    Blog Post
    ...CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions describing...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  20. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...families, eating delicious food, or playing one of the giant games or indoor mini-golf course.    While the main...
Showing: 941 - 960 of 1085 results