We narrowed to 1,019 results for: CAL;
-
TypeBlog Post...guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than the RNA guides employed by...
-
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post... that will bring about the next revolution in biological research. We’re incredibly happy to see that ... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...incredibly useful, yet simple system allows you to systematically study gene function with temporal control and... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post... or pink. Purpose Gram staining can be used in medical settings to determine the general type of bacteria... -
How to Lead a Great Meeting
TypeBlog Post...ensures that everyone gets a chance to talk and less vocal team members still have their voices heard. Here's... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the best... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... -
Gamma-Retroviral Vector Guide
TypeGuide..., whereas gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses...altered by changing the envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope...commonly used in gene therapy applications to more specifically direct delivery of gene therapies. Common Uses...gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression of ...protocols for fast and efficient generation of large-scale, high-titer preps. This is thanks to established...lines that are able to keep up with the demand of clinical studies and commercial purposes. The use of helper-free... Khinder, R., . . . Pule, M. (2022). Efficient clinical-grade gamma-retroviral vector purification by ... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...antibiotic-containing agar. This protocol will focus specifically on selection with carbenicillin; however it ...required for effective over-agar selection has been empirically determined. See selection curve below. Carbenicillin... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...microfuge tube. Using a scale, weigh the tube with the gel fragment after zeroing the scale with an empty tube... -
Water Bath Protocol
TypeProtocol...materials to a particular temperature, catalyze chemical reactions such as restriction digests needed in...sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops of the tubes... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post... disciplinary communities gather at ComSciCon's local and specialized franchise workshops throughout the... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing! A recent... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target... -
Targeted Mutagenesis with EvolvR
TypeBlog Post... and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...in target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...m always catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...need to determine these transduction patterns empirically for their own experiments. To this end, we offer... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...