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... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid. A variety of...

...this system was optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets. When it’s ready to transform...

...Science Careers’ myIDP tool. Using myIDP, you can identify suitable careers based on your current interests...anxiety of graduate and postdoc training. On the other side, the PI has an opportunity to check in with lab ...

... own unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes... describe a modular collection of plasmids for a wide variety of genome modifications in S. Pombe, including...

...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...

...respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive, high-level...

...dramatically increase the chances of being seriously considered for an open position.  Check out Joanne's Reddit...network your way to a hiring manager. Learn to confidently describe your strengths in your resume and don't...

...Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs, Kristin ...about academia and alternate training needs to be provided to take advantage of all the possible science ...

...way to share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my...scientific discovery or invention, and sharing those ideas with others. This love of interacting with people...

...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...

...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method...

...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods...

...well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ...

...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running on a time crunch...

...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ...

...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...