We narrowed to 972 results for: arc

... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...

... Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv...

...more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements...

...ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014...

... with Linkers (MODAL) strategy." Nucleic acids research 42.1 (2014): e7-e7. PubMed PMID: 24153110. PubMed...

...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...

... that you can make on your own.  To narrow your search, first pay attention to the validated applications...

...numbered AAV1, AAV2, and so on. In addition, researchers have created many engineered serotypes by mixing...

...the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express random...

...pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting time to be in ...

...blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine...

...of tissue clearing techniques (9) now allows researchers to image tissues that are over a cm3 in size....

...design your first flow panels! Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...

...and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly...

...modifications, we recommend doing a little more research into gel chemistry and/or specialty gel options...

...peak action spectra Example: Jaws Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...sensitivity Example: ArchT Increased photocurrent amplitude Examples: eArch3.0, eArchT3.0 Mac Leptosphaeria...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering to improve these...have been instrumental in neurological disorder research, helping scientists to better understand Parkinson's...

... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...Overview of Cas9n. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert... 5: Overview of base editing. More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

... a single tube. Types of Pooled Libraries Barcoding Barcode libraries contain large numbers of plasmids...nucleotide sequence, or barcode, used to mark individual cells. These barcodes will then be inherited ...any daughter cells, making barcode libraries ideal for lineage tracing. Barcode libraries can be used for...design additional hypothesis-directed experiments. Barcoding libraries contain plasmids with unique, semi-random...designed for other specific purposes, such as barcoding or targeting RNA. Advantages Strong, specific ...shRNAs that target it. shRNA libraries may also be barcoded to allow for easy identification of the shRNA ...positive and negative) is the responsibility of the researcher. Created with BioRender.com. Both positive and...

...conducting cellular barcoding experiments. Lentiviral vectors allow for integration of the barcodes, which can ...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic screens, lentiviral vectors ...inherited by daughter cells. Inheritance of the barcodes makes lentiviral libraries popular for lineage...heterogeneous tumors. Browse Addgene's collection of barcoding libraries . Mouse Modeling Since the development...

...Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral (rAdV) vectors...gene expression patterns that were observed, researchers have tended to favor using first- and third-generation...is still the most commonly used generation for research purposes. The system consists of two plasmids ...in combination with foreign capsid proteins. Researchers have further refined the tropism of rAdV vectors...vectors are often the preferred choice among researchers due to their reduced immunogenicity and longer...transgene expression, rAdV vectors are used in many research fields, especially when needing transgenes to ... and can trigger a strong immune response to researchers if exposed. There is a risk of spontaneous reversion...