We narrowed to 969 results for: arc

...ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014...

... with Linkers (MODAL) strategy." Nucleic acids research 42.1 (2014): e7-e7. PubMed PMID: 24153110. PubMed...

...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...

...numbered AAV1, AAV2, and so on. In addition, researchers have created many engineered serotypes by mixing...

... that you can make on your own.  To narrow your search, first pay attention to the validated applications...

...the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express random...

...blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine...

...pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting time to be in ...

...design your first flow panels! Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...

...of tissue clearing techniques (9) now allows researchers to image tissues that are over a cm3 in size....

...and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly...

...modifications, we recommend doing a little more research into gel chemistry and/or specialty gel options...

...peak action spectra Example: Jaws Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...sensitivity Example: ArchT Increased photocurrent amplitude Examples: eArch3.0, eArchT3.0 Mac Leptosphaeria...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering to improve these...have been instrumental in neurological disorder research, helping scientists to better understand Parkinson's...

... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...Overview of Cas9n. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert... 5: Overview of base editing. More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

...conducting cellular barcoding experiments. Lentiviral vectors allow for integration of the barcodes, which can ...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic screens, lentiviral vectors ...inherited by daughter cells. Inheritance of the barcodes makes lentiviral libraries popular for lineage...heterogeneous tumors. Browse Addgene's collection of barcoding libraries . Mouse Modeling Since the development...

...Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral (rAdV) vectors...gene expression patterns that were observed, researchers have tended to favor using first- and third-generation...is still the most commonly used generation for research purposes. The system consists of two plasmids ...in combination with foreign capsid proteins. Researchers have further refined the tropism of rAdV vectors...vectors are often the preferred choice among researchers due to their reduced immunogenicity and longer...transgene expression, rAdV vectors are used in many research fields, especially when needing transgenes to ... and can trigger a strong immune response to researchers if exposed. There is a risk of spontaneous reversion...

...Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated viral ...in combination with foreign capsid proteins. Researchers have further refined the tropism of rAAV vectors...diffused biological structures. Specifically, researchers were interested in creating systemic capsids ...send information to a given region. This allows researchers to trace connections between neurons, map the...make them ideal for gene therapy, neuroscience research, and other biomedical fields. Gene Therapy One... opsins and other optical tools developed by researchers are used to detect, measure, and control molecular...institution's best practices with regard to AAV-related research. See Addgene’s Biosafety Resource Guide for more...

...types include murine leukemia virus (MLV), murine sarcoma virus (MSV), and feline leukemia virus (FeLV). ...treatments into cells, and has been widely used in the research community for decades. Wildtype gamma-retroviruses...vectors have many aspects that make them useful research tools in a variety of cell types and models, such...integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression...practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide for more...Retroviral Vectors and Their Uses in Scientific Research Gamma-retroviral Packaging Systems The Retroviral... enhancers and active promoters. Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org/10.1093/nar...

...enables researchers to manipulate the genome with relative ease. Over the past decade, researchers have ...are many different Cas proteins available, and researchers can adapt these experiments for different organisms...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus...proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with your PI to ...

...codon-amino acid pairs. Over the past two decades, researchers have also expanded the genetic code by redirecting...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC...fragmented into small pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached...information to aid in the reproducibility of scientific research. Long-read Sequencing Sanger sequencing and Illumina...