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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 969 results for: Cre

Showing: 961 - 969 of 969 results

  1. Molecular Cloning Techniques

    Type
    Guide
    ... the gap created by the 5’ exonuclease. Finally, ligase seals the nicks in the DNA to create one piece... Stitching Resources Molecular cloning, or the creation of recombinant DNA, is an essential process used...several of the most popular cloning methods for the creation of recombinant DNA. Restriction Enzyme Cloning... of interest (insert) into a plasmid backbone. Created with BioRender.com. Gateway Recombination Cloning...compatible attP sites via a proprietary BP clonase, creating an entry clone. The entry clone now has recombined...blog post . Figure 2: Summary of Gateway cloning. Created with BioRender.com. TOPO Cloning Toposiomerase-... enzymes or an exogenous ligase, providing an incredibly quick and easy way to clone a fresh PCR product...

  2. Plan Your Experiment

    Type
    Guide
    ... mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less ...efficiency. In fact, cleavage efficiency may increase or decrease depending upon the specific nucleotides ... the general framework of a CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic...mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying ...likely won’t be able to completely avoid them. To increase specificity, consider using an engineered Cas ...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  3. Modular Cloning Guide

    Type
    Guide
    ...) to optimize secretion and cell surface display of proteins of interest. Yeast Secrete and Detect Yeast...It is a powerful and very efficient method for creating many plasmids from different combinations of a... directionally assembled into a Level 1 vector creating a single transcriptional unit (for example, a ...Combining multiple Level 2 vectors permits the creation of even more complex constructs constrained only...for cloning and assembling standardized parts to create eukaryotic multigene constructs. MoClo Plant Parts...System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several cassettes...with additional Level 0 modules and components to create plasmids with multiple transcriptional units. MultiGreen...

  4. Antibody Guide

    Type
    Guide
    ... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...scientists have made changes to these components for increased safety when producing viral vectors in a laboratory.... Figure 1: Wild-type gamma-retrovirus genome. Created with BioRender.com. All gamma-retroviral vectors...distinct plasmids for safety to reduce the chance of creating replication-competent retroviruses. For a summary...tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral Vector Production... 3: Gamma-retroviral vector packaging methods. Created with BioRender.com. Viral Vector Integration Much...genome and the cells that survive can be expanded to create stable cell lines. The cell lines will have the...transcription. Removal of this region in the 3’ LTR creates self-inactivating viral vectors. R in cis Repeat...

  6. Promoters

    Type
    Guide
    ...Simplified promoter region during transcription. Created with BioRender.com. DNA sequences called response...bacterial promoter. TSS = transcription start site. Created with BioRender.com. Operons Although bacterial ...positively-controlled operon. Figure 3: Lac operon regulation. Created with BioRender.com. Common Bacterial Promoters ...transcription factor; TSS = transcription start site. Created with BioRender.com. Core Promoter The core promoter...

  7. Educational Resources

    Type
    Guide
    ...Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests ...

  8. Science Guides

    Type
    Guide
    ... Science Guides Addgene has created the following guides to help you start learning...

  9. Addgene's eBook Collection

    Type
    Guide
    ...and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent Proteins...
Showing: 961 - 969 of 969 results