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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 967 results for: Cre

Showing: 961 - 967 of 967 results

  1. Cloning

    Type
    Guide
    ...the gap created by the 5’ exonuclease and finally ligase seals the nicks in the DNA to create one piece...Cloning Oligo Stitching Molecular cloning or the creation of recombinant DNA is an essential process used...several of the most popular cloning methods used to create recombinant DNA. Restriction Enzyme Cloning Restriction...compatible attP sites via a proprietary BP clonase (creating an entry clone). The entry clone now has recombined...restriction enzymes or an exogenous ligase providing an incredibly quick and easy way to clone a fresh PCR product...available TOPO® ready, and it is not feasible to create a TOPO® vector yourself. Additionally, the efficiency...distal to their recognition site allows for the creation of custom overhangs, which is not possible with...

  2. Antibody Guide

    Type
    Guide
    ... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...scientists have made changes to these components for increased safety when producing viral vectors in a laboratory.... Figure 1: Wild-type gamma-retrovirus genome. Created with BioRender.com. All gamma-retroviral vectors...distinct plasmids for safety to reduce the chance of creating replication-competent retroviruses. For a summary...tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral Vector Production... 3: Gamma-retroviral vector packaging methods. Created with BioRender.com. Viral Vector Integration Much...genome and the cells that survive can be expanded to create stable cell lines. The cell lines will have the...transcription. Removal of this region in the 3’ LTR creates self-inactivating viral vectors. R in cis Repeat...

  4. Molecular Biology Reference

    Type
    Guide
    ...study DNA fragments of interest, such as genes. Creation of recombinant DNA Molecular Cloning Plasmids ... With current cloning technology, it is easy to create and modify plasmids containing the genetic element...vector can also include an enhancer sequence which increases the amount of protein or RNA produced. Expression...into target cells. You can use these plasmids to create viral particles, such as lentiviral, retroviral...used in place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise...

  5. Educational Resources

    Type
    Guide
    ...Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests ...

  6. Science Guides

    Type
    Guide
    ... Science Guides Addgene has created the following guides to help you start learning...

  7. Addgene's eBook Collection

    Type
    Guide
    ...and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent Proteins...
Showing: 961 - 967 of 967 results