We narrowed to 144 results for: transfection

...mammalian selection cassette to screen for positive transfected cells. The addition of an in-frame fluorescent...you can get away with generating transiently transfected cells, you will not need a mammalian selection...

...constitutively expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome... to cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid...frequency of base edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter ...

...interference (RNAi). The most common method is transfection of short interfering RNA (siRNA). Knockdown .... This approach often involves the transient transfection of expression plasmids in a given cell line....antibody should be able to detect even in cells transfected with low efficacy. But since there can be large...differences between endogenous expression and transfected overexpression, this approach cannot confirm ...

... number of plasmids that are required in the transfection step of the viral packaging cell line. For example...resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide  ...supplied by three types of plasmids, which are cotransfected into the 293T packaging cell line. This system...

...base pairs on each side, in fact. Later, when we transfect the cells, these large regions of homology enable...being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing...

... your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time ...the correct transfer plasmid was used in the transfection; if preparing several different lentiviruses...

...ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent proteins...measure (overexpressing a gene or protein after transfection). Future of FRET The first genetically encoded...

...BiFC constructs are expressed transiently by transfection or stably impacts the success of BiFC experiments...dose-dependent manner. This is straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly...

...in mammalian cells is relatively quick. After transfection, HEK-293T cells start producing virus within...systems, it is standard to do only one passage (transfection). This differs from typical insect cell preparations...

...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...