We narrowed to 772 results for: LIS
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TypeBlog Post... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which... -
Hot Plasmids - November 2023
TypeBlog Post...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D.... -
Design Tips for Prime Editing
TypeBlog Post...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021 Yujie Sun ... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds to the promoter... -
Using AAV for Neuronal Tracing
TypeBlog Post...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R... -
mTOR Pathway
TypeCollection...PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3)...Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...David Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...saturate the detector. In between these extremes lies the linear detection range. Figure 4: The... -
Hot Plasmids: Fall 2024
TypeBlog Post...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...-4 Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion... -
Plant Plasmids and Resources
TypeCollection...editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
CRISPR Pooled gRNA Libraries
TypeCollection...Transcription Factor Knockout Library 162275 Knockout Human Li 3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries...UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195 Knockout Human...different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249 Knockout Mouse...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~... -
CRISPR History and Development for Genome Engineering
TypeCollection..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015....mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection... Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem Cell . 2013. PubMed . Electroporation...