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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 885 results for: TRI

Showing: 81 - 100 of 885 results

  1. Open Enzyme Collection

    Type
    Collection
    ...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

  2. Cre-lox system

    Type
    Collection
    ...a promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...Muscle-specific expression of Cre MCK Mammalian Kahn 13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake...puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre iRFP670, Cre, and GFP ...Luo 119063 pBac(3xP3-gTc’v; Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle...Ohlmann 120279 014LP-pAAV-ANF-657- Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre... partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Read the...

  3. Plan Your Experiment

    Type
    Collection
    ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested...digested with the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR...

  4. Bacterial Expression Systems

    Type
    Collection
    ...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible ...YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible ... and P. pastoris . All plasmids use the same restriction enzymes for cloning so multiple constructs can...compatible with standard cloning using many different restriction enzymes. pPro24-gfp contains GFP but all others...

  5. CRISPR Guide

    Type
    Collection
    ...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

  7. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

  8. AAV Viral Preps

    Type
    Collection
    ... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde...

  9. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...as in-stock preps. These high-titer preps are distributed as 100 µL solutions of purified viral particles...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

  10. Microbiology Resources

    Type
    Collection
    ...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations... pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50 75026 Worm...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...flow strips that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm...lateral flow strips. Since the probes are tagged with streptavidin, the lateral flow strip first captures...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-... this virus has become an utmost priority for countries around the world and to do so, the WHO recommends...testing, tracking, and social distancing. With countries like South Korea, one of the earlier epicenters...be captured by an antibody further along on the strip. Highlights: Of the 83 known COVID-positive samples...viruses. Unlike Cas12a, Cas13a does not require strict sequence preferences at the target site, while ...

  14. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...’s catalog — from the recently developed to the tried-and-true classics. Check out our Biosensors Collection...biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s...TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent protein is inserted... ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT enabled real-time observations of oxytocin dynamics...earlier tools. They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters...ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal...sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...get them below a given threshold even when you’ve tried everything listed here. For some applications, this...CRISPR to (hopefully) be used in human clinical trials. An off-target rate of 5% is far too high when ...

  16. COVID-19 Resources

    Type
    Collection
    ...Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids. The collection contains:... a for-profit entity, please see our Plasmid Distribution to Industry page. The list of plasmids available...Cathepsin L - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...131779 pAAV-nEF-Con/Fon TVA-mCherry Cre AND Flp No Triple recombinase-dependent Addgene ID Plasmid Logic ... evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786... Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours),...Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral ...Tet-Off and additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1 Lentiviral...

  19. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs tagged...found below. In addition, the Davidson lab has contributed the corresponding empty backbone with different...

  20. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...plasmids available to scientists in academic and industrial organizations. For more information on ordering... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...
Showing: 81 - 100 of 885 results