Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 400 results for: pho;

Showing: 121 - 150 of 400 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...started!   Thanks for all your hard work!   Fluorophores When is a Monomer not a Monomer? The Top Three...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments...experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based machines...

  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  4. Distribution to Industry

    Type
    Collection
    ...through our website. We cannot accept orders by phone, fax, or email. Pricing Addgene is a nonprofit organization...

  5. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests...have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most...with colleagues and link your notebook from your smartphone to your computer. Lab Archives will help both...

  6. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...questions. Don't try to get all panelists on the phone at once – you won't get as much out of it and it...better at remembering these things on a relaxed phone call than when first confronted on a stage in front...front so the interactions are better. Have a good microphone system ready for each panelist if the room needs...what happens to the attention of the audience – phones come out, postures shift, they are checking out...

  7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to... coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000). Chromoproteins also enhance... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P)...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...dTomato, displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et ...

  12. Open Enzyme Collection

    Type
    Collection
    ... DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources Bacterial...

  14. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and helper...consistently shown promise in reducing leukemia and lymphoma and are in their clinical trial phase. In all ... this results in frequent physiological and morphological changes. Tumors differ in their genotypic and...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

  18. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...often used to monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow... Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources...

  19. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited to autophagasomal membranes...Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology...protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources...

  20. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...

  22. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  25. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...grow over time, but being interested in the same phosphate of your favorite kinase is a good start. Check...next to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...meals, sit with people you don’t know and put your phone away (did I say that already). Start with, “Is this...

  26. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  27. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  29. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCTGATACTCCTGCTGGtggcgtcccgtctggaTGCTGTGAGCTTTCCTGA Semaphorin 6bb TAL3356 & TAL3357 TTGAAAAGTGCTGAGAAAtgtgatgtcattggagTTTGATTTAGGAGACAAA...
Showing: 121 - 150 of 400 results