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We narrowed to 189 results for: lentiviral

Showing: 161 - 189 of 189 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  2. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  3. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  5. Viral Vectors

    Type
    Collection
    ...engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses are versatile tools because of...Target Cell Genome Integration Immune Response Lentivirus Stable RNA <8 Kb 80-130 Dividing/Non-dividing...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  8. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  22. Lab to Office Culture Shock

    Type
    Blog Post
    ...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize...

  23. Plasmid Collections

    Type
    Collection
    ...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  26. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  27. Immunology Research Plasmids and Resources

    Type
    Collection
    ...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...

  29. Science Guides

    Type
    Guide
    ...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...
Showing: 161 - 189 of 189 results