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We narrowed to 376 results for: fus

Showing: 201 - 250 of 376 results
  1. CRISPR Cheat Sheet

    Type
    Blog Post
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...
  2. Chemogenetics AAV Preps

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    Collection
    ...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...mCherry fusion none 5 Bryan Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50455...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50458...-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry fusion Cre-dependent 8, rg* Bryan Roth 50474 pAAV-hSyn-hM3D...
  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further... and of the fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein...MBP-TEV N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in ...vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more...insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible... by: Species-Specific Expression Epitope Tag or Fusion Protein Function: Viral Vector Delivery, Genome... vectors - Inducible expression, fusion proteins, and more pGBKT7-GW - Gateway...
  4. Editor's Choice, August 2016

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    Blog Post
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...
  5. Luciferase Plasmid Collection

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    Collection
    ...LumiScarlet : A fusion of LumiLuc (ATP-independent luciferase derived from teLuc) fused to mScarlet-I. ..., Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale...pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-...
  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...
  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...
  8. Antibodies 101: Epitope Tags

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    Blog Post
    ... References Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to...
  9. Plasmid Cloning by PCR

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    Blog Post
    ...in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer...
  10. Promega Plasmid Collection

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    Collection
    ...vectors for sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag... sensitive gene expression analysis and protein fusion vectors for detecting and tracking proteins using...high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link opens in a new window) is a 19.1 ...detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular and extracellular...protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in a new window) technology...puts control of the function and properties of a fusion protein in your hands, thanks to interchangeable...PPI) studies, where each protein of interest is fused to either LgBiT or SmBiT and expressed in live cells...
  11. The MTA at Addgene

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    Blog Post
    ....  Why the MTA?  The MTA process can be a bit confusing, and it’s certainly frustrating to find out your...
  12. Fluorescent Protein Guide: Subcellular Localization

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    Collection
    ...example, if the marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...has assembled a collection of popular fluorescent fusion proteins that are targeted to various organelles...well-characterized marker's localization pattern, you should fuse your protein of interest with a fluorescent protein...Primary cilia (Neurons) Drd1 EGFP Kirk Mykytyn *Fusions to other FPs can be found by searching the complete...microtubule-binding domain mCherry Maria Harrison *Fusions to other FPs can be found by searching the complete...
  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques...
  14. TALEN Plasmids and Kits

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    Collection
    ...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...for expression as TALE-LSD1 fusions. It was recently shown that TALE fusions with the histone demethylase...are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors...Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter... Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...Visualization [TGV]). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be...
Showing: 201 - 250 of 376 results