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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 109 results for: 1-Oct

Showing: 81 - 100 of 109 results

  1. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...occur, but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer... and 1 x 1012 vg*. Most systemic capsids are easy to produce in high titer, but higher doses (>1 x 1012...before using it in an application.     Figure 1: AAV toolkit for targeted gene delivery to the brain...decision tree for selecting a capsid, while Table 1 gives a brief overview of published descriptions of...Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids available from Addgene ...AAV9. At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater...Diverse Brain Cells. Annual Review of Neuroscience, 45(1), 447–469. https://doi.org/10.1146/annurev-neuro-111020...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...detects samples at clinically relevant concentrations (1-3 fM; 1x106-fold lower than before), and our combined... health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection ... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...

  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... to the development of resistance include (Figure 1): Over-prescription of antibiotics (for example, ... communication, and public health.     References 1. O'Neill, J. "Tackling drug-resistant infections globally...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...

  5. dTAG - You're it!

    Type
    Blog Post
    ...cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated a series of plasmids that...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and...genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

  7. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

  8. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

  9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ...Max Planck Society. Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the ...

  10. March for Science

    Type
    Blog Post
    ...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...

  13. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...breaks (DSBs) or donor DNA templates.  Figure 1: An overview of prime editing. Created with BioRender.com...demonstrated ~20-50% efficiency in HEK293T cells with 1-10% indels. Since then, further innovations have continued...Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...

  14. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to.... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...

  15. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...Bioinformatics from Boston University, and did postdoctoral work at Harvard Medical School and the University...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...candidates for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went...

  17. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...regarding what makes this group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

  19. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize every opportunity prior...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

  20. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...Guerin and Susanna Stroik. Didem Goz Ayturk is a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal...
Showing: 81 - 100 of 109 results