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We narrowed to 347 results for: ATC

Showing: 81 - 100 of 347 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed is plated...scanned into our LIMS system to ensure that they match, helping us to avoid mixing up samples as we transform...process, we ensure that the sample tubes that we use match those of the appropriate deposit. Using barcoded...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts. (B...four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate. PRO, promoter...create the resulting plasmid map and identify any mismatches that are preventing your overhangs from aligning...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...not function properly. This is the result of a mismatch between human and E. coli codon preference. Some... abundances of the codons I choose for the host match the abundances of codons used in the native organism... constructs. However, due to problems such as mismatched translation rates and potential effects on cell...

  5. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look...2012, for example, 3 billion hours of video were watched each month, in 39 countries, and in over 54 languages... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...would ease people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...more important lab work from being done. You can watch the first video in our Intro to the Lab Bench series...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...mutation to match a frequent mutation in early-onset GC and one with a mutation at R273H to match a mutation...EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins involved...

  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... determine optimal DOL is through several small-batch experimental labeling. Size is also a factor. Bulkier... wavelengths and emission profiles, need to be matched with the experimental setup and objectives. Most... technical manuals. Specific applications and matching dye/ligand choices For effective live-cell imaging...

  10. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...checked. Assuming full complementarity, its sequence matches the guide sequence. PAM: The “protospacer-adjacent... and target in order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion...

  11. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...Mammalian Expression Systems and Delivery Methods and A Match Made in Heaven: CRISPR and AAV Day 6: Bacteria: ...

  13. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...in your research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral... and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and...the reaction mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology ...

  19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter set, ... emission spectra are red. Neither is a perfect match to the filter set but the excitation filter excites...

  20. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... neuronal activity, scientists can use GECIs to watch neurons fire under the microscope. To add another... usefulness depends on selecting a fluorophore matched to their excitation wavelength. OCaMP is well-suited...
Showing: 81 - 100 of 347 results