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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 410 results for: ATC

Showing: 81 - 100 of 410 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...team in the UK is five hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation... team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications with my UK team members...out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when I ...

  2. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...networker (or if you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships... the country with groups who are piloting this. Watch for my upcoming mentoring post in which I describe...naturally out of friendships or work interactions. Watch for the people that are already teaching you and...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... “copies.” This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies,...to non-target proteins), variability (separate batches can perform differently – happens when the antibody...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    ...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences...

  6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up...genetic cross). You can tell when pupae are ready to hatch the next day because the pupae will develop a visible...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences...

  9. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... “copies.” This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies,...to non-target proteins), variability (separate batches can perform differently – happens when the antibody...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of a 30 nt target...edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed... targeted nucleotide. Introduction of guanine-mismatches in the guide across from these off-target edits...

  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed is plated...scanned into our LIMS system to ensure that they match, helping us to avoid mixing up samples as we transform...process, we ensure that the sample tubes that we use match those of the appropriate deposit. Using barcoded...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

  13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...in the previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this...available filters to refine your search results to match your experimental needs. Remember, only filters ...insert, or use the other options to find plasmids matching your search terms with gene and guide RNA / shRNA...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...not function properly. This is the result of a mismatch between human and E. coli codon preference. Some... abundances of the codons I choose for the host match the abundances of codons used in the native organism... constructs. However, due to problems such as mismatched translation rates and potential effects on cell...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look...2012, for example, 3 billion hours of video were watched each month, in 39 countries, and in over 54 languages... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

  16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts. (B...four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate. PRO, promoter...create the resulting plasmid map and identify any mismatches that are preventing your overhangs from aligning...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

  18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts. (B...four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate. PRO, promoter...create the resulting plasmid map and identify any mismatches that are preventing your overhangs from aligning...

  19. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...would ease people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...more important lab work from being done. You can watch the first video in our Intro to the Lab Bench series...
Showing: 81 - 100 of 410 results