...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

...detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed is plated...scanned into our LIMS system to ensure that they match, helping us to avoid mixing up samples as we transform...process, we ensure that the sample tubes that we use match those of the appropriate deposit. Using barcoded...

...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look...2012, for example, 3 billion hours of video were watched each month, in 39 countries, and in over 54 languages... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

...not function properly. This is the result of a mismatch between human and E. coli codon preference. Some... abundances of the codons I choose for the host match the abundances of codons used in the native organism... constructs. However, due to problems such as mismatched translation rates and potential effects on cell...

...would ease people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...more important lab work from being done. You can watch the first video in our Intro to the Lab Bench series...

...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole ...own research needs. Other companies like NEB and ATCC can provide you with additional validated reagents...

... determine optimal DOL is through several small-batch experimental labeling. Size is also a factor. Bulkier... wavelengths and emission profiles, need to be matched with the experimental setup and objectives. Most... technical manuals. Specific applications and matching dye/ligand choices For effective live-cell imaging...

...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...Mammalian Expression Systems and Delivery Methods and A Match Made in Heaven: CRISPR and AAV Day 6: Bacteria: ...

...checked. Assuming full complementarity, its sequence matches the guide sequence. PAM: The “protospacer-adjacent... and target in order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion...

...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

...in your research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral... and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...

...(2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations....

...single mismatch at any position of the DNA guide impaired NgAgo activity, with three mismatches abolishing...

...developing embryos are transparent, so it’s easy to watch development in real time.  In both cases, dL5** ...light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages of MG-2I-dL5** over other...

... pathways. Individual cellular fates were then matched with candidate TFs responsible for that differentiation...collection, we’re releasing individual plasmids in batches. If there’s something you’d like that’s not yet...

...polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and...the reaction mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology ...

...efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter set, ... emission spectra are red. Neither is a perfect match to the filter set but the excitation filter excites...

...Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...

...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session on our YouTube ...

... If that’s not enough, we consult with experts, watch webinars, or use other research methods. In the...repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making content...