...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...

...requested through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All ...terminators, promoters, and genes) were built with flanking primer binding sites (designed to have 20-30 nt...parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting...characterization of the recombinases at our disposal.  Many thanks to our guest blogger Nathaniel Roquet! Nathaniel...

...Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond Gosling took the first...diffracted image of the DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved...Inferno (2013) and the movie version (2016) stars Tom Hanks as the protagonist (Langdon). The plot involves ...?Download high resolution versions here.  Many thanks to our guest blogger Kartik Lakshmi Rallapalli ...

...primers that amplify the DNA sequence of interest and flank it with 50 base pairs of homology to the targeted...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...limited circumstances. An alternative approach is to flank the desired change with 4-5 silent changes in the...recombineering system? Share your tips below! Many thanks to our guest blogger, Beth Kenkel Beth Kenkel is...

...cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo...uDEE9PsspD#deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com/DWKWaPs0iY— Xi Chen...  Congratulations to all our winners, and a huge thank you to everyone who decked their lab and brought...

...importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth Fan, and Benjie...Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in ...Engineering from MIT, both in Computer Science.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

...Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location: 75 Sidney...for our favorite people – you, our scientists!  Thank you for being part of our growing community! Our...

...accessible for scientists as possible.  He also thanked our many business partners, without whom Addgene...essential advice and service. Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first...family, too. Here’s to another great 10 years!    Thank you to our guest blogger! Blugene, Addgene's friendly...

... genuine pleasure to send these small tokens of thanks for the incredible impact our depositors have had...particular our Blue Flame Award winners- we’d like to thank you for sharing your plasmids at Addgene. You are... we’re able to do what we do!          Special Thanks Trophies and engraving services from Hit Trophy...

... getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier is the Chief Scientific Officer...members to the Board of Directors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to...

... of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence the nomenclature...endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many immature neuron-...great model of kidney cell biology, there are thankfully many other applications that they are useful ...

... insert of interest. The transgene sequence is flanked by long terminal repeat (LTR) sequences, which ... between vectors until the gene of interest is flanked by sites compatible with those in the vector into... first cloned into an entry vector at a region flanked by sequences (called attP1 and attP2) that allow...

...of the De-jargonizer.   Academic Phrasebank  The Academic Phrasebank is a resource for phrases commonly...being critical, or defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit...

...s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who helped you succeed...any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect...helped someone get a job interview. Even just a thank you email would have been enough, but I’ll never...

...control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning...to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite...related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt (left) and...

...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...different emission filters) or different set-ups. Many thanks to our guest bloggers Joachim Goedhard and Marieke...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt is a graduate...

...the Max Planck Institute for Brain Research in Frankfurt am Main, Germany. She presented a novel technology...to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing...endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas! Eugenia Rojas...

...several human and murine CRISPR pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long...constructs, and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal is is now ...vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy cloning of...revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided targeting...difficult to quantify interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular...

...sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh family, we ...completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of a pipeline...labs often provide insert sequences or annotated Genbank files that are useful for more complicated plasmids...