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We narrowed to 499 results for: Ank

Showing: 81 - 100 of 499 results

  1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...two sets of sgRNAs; A, the top 3 ranked sgRNAs, and B, the next 3 ranked sgRNAs. Dolcetto was able to discriminate... lab selected sgRNAs in this optimal window and ranked them based on their optimized sgRNA rules and potential... and Dolcetto library, sgRNAs were selected and ranked based on the previously established sgRNA rules...

  2. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...requested through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All ...terminators, promoters, and genes) were built with flanking primer binding sites (designed to have 20-30 nt...parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting...characterization of the recombinases at our disposal.  Many thanks to our guest blogger Nathaniel Roquet! Nathaniel...

  3. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond Gosling took the first...diffracted image of the DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved...Inferno (2013) and the movie version (2016) stars Tom Hanks as the protagonist (Langdon). The plot involves ...?Download high resolution versions here.  Many thanks to our guest blogger Kartik Lakshmi Rallapalli ...

  4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...primers that amplify the DNA sequence of interest and flank it with 50 base pairs of homology to the targeted...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...limited circumstances. An alternative approach is to flank the desired change with 4-5 silent changes in the...recombineering system? Share your tips below! Many thanks to our guest blogger, Beth Kenkel Beth Kenkel is...

  5. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo...uDEE9PsspD#deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com/DWKWaPs0iY— Xi Chen...  Congratulations to all our winners, and a huge thank you to everyone who decked their lab and brought...

  6. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth Fan, and Benjie...Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in ...Engineering from MIT, both in Computer Science.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  7. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location: 75 Sidney...for our favorite people – you, our scientists!  Thank you for being part of our growing community! Our...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...accessible for scientists as possible.  He also thanked our many business partners, without whom Addgene...essential advice and service. Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first...family, too. Here’s to another great 10 years!    Thank you to our guest blogger! Blugene, Addgene's friendly...

  9. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ... genuine pleasure to send these small tokens of thanks for the incredible impact our depositors have had...particular our Blue Flame Award winners- we’d like to thank you for sharing your plasmids at Addgene. You are... we’re able to do what we do!          Special Thanks Trophies and engraving services from Hit Trophy...

  10. Meet the New Addgene Board Members!

    Type
    Blog Post
    ... getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier is the Chief Scientific Officer...members to the Board of Directors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to...

  11. What the HEK?

    Type
    Blog Post
    ... of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence the nomenclature...endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many immature neuron-...great model of kidney cell biology, there are thankfully many other applications that they are useful ...

  12. Adenovirus Guide

    Type
    Guide
    ...and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats (ITRs). Adenoviral...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293 or HER911...genome packaging. In helper viruses, this signal is flanked by loxP sites so they can be excised. Adenoviral...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning...to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite...related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt (left) and...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who helped you succeed...any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect...helped someone get a job interview. Even just a thank you email would have been enough, but I’ll never...

  16. Lentiviral Vector Guide

    Type
    Guide
    ... vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything in between will...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid...replication-competent lentiviral vectors is very low thanks to the design of second- and third-generation lentiviral...

  17. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...different emission filters) or different set-ups. Many thanks to our guest bloggers Joachim Goedhard and Marieke...

  18. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt is a graduate...

  19. CRISPR Guide

    Type
    Guide
    ...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...the Max Planck Institute for Brain Research in Frankfurt am Main, Germany. She presented a novel technology...to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing...endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas! Eugenia Rojas...
Showing: 81 - 100 of 499 results