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We narrowed to 201 results for: Arch-GFP

Showing: 81 - 100 of 201 results

  1. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    .... Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative Meidince - Understanding and...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was... but later got obsessed with research. Chapter 3: How all basic research ends up being translational. ...for Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk, ...at Boston University Medical School. Darrell’s research focuses on the lungs, and, among other projects...Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell recently...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a single gene to cells. ...crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as ...we decided to write this quick guide to help researchers choose the probe for their needs. You’ll find...for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility to be used as internal tags due... normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was...biosensors, and optogenetic tools. Follow her recent research @daria_gift_shch.     References and resources...

  3. Brain Initiative Collection

    Type
    Collection
    ...115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter... pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...

  4. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ... lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9 and further...2016 was an exciting year for genome engineering research. A variety of new tools came out including the...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series of HEK 293T cells expressing...membrane-targeted SuperPLDx30 (magenta) and PA probe GFP-PASS (green). After 30 minutes of blue light illumination...more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements...

  6. Adenovirus Plasmids

    Type
    Collection
    ... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...regions present in pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral A block for the AdenoBuilder genome assembly...system. Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle ...Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene under a chosen...beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer Vogelstein 18703...

  7. Retrovirus Plasmids

    Type
    Collection
    ...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro.... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression...Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional... retroviral plasmid with your gene of interest? Search our database for retroviral transfer plasmids that...of dsRed and the activation of your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV...

  8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...collection of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...to either the N-or the C-terminus. Even if a researcher’s gene of interest isn’t in the ORF collection...in the Davidson lab for the last four years. “Researchers can make their own target if it is not already...can essentially cut and paste quite quickly. Researchers should keep in mind that a very simple sequential...deposit the tools he and his team created with the research community at large, "[Fluorescent proteins are...

  9. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell... PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control...pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Boyden 59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Inhibitor...Recombinases Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre expression Recombinases...Cre-dependent Optogenetics Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CamKII Inhibitor Optogenetics Boyden ...Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Roth 114472...

  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process...genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...is a component of the immune system useful as a research reagent? Let’s find out! What is an antibody? ...system and a tool that can be used for a variety of research applications. Within the immune system, antibodies...ability of antibodies to bind proteins is useful for research applications as well because they allow scientists...and initiates different immune cascades. In the research setting, antibodies of different isotypes can ...antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to spot the whole...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...

  13. Brain Armamentarium

    Type
    Collection
    ...James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway...pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple...expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues to work...sensors was developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019).... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...

  15. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered...animals have impacted humans through biomedical research. The Camelid family is composed of camels, llamas... llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of ...experiments, due to their simple structure    sdAbs in research These advantages have contributed to sdAbs becoming...variable domains (aka nanomice), which gives researchers the “best of both worlds” - a familiar lab model...

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds us that, although fluorescent proteins.... To learn how you can use minigenomes in your research, check out this post! R Bodies: Membrane-Rupturing... post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at Harvard University, describes...Genome Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits...In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better...Addgene Blog useful resources for the biological research community! ...

  17. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce...this important agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that...applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil bacterium...groups of scientists working in a wide range of research fields and geographical locations. We are excited...

  18. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ...also bring benefits to researchers conducting basic or early pre-clinical research. However, standard terms...concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing and improving...resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say...

  19. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community.    So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ...

  20. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature... Produce synthetic viruses that can be used in research or for therapeutics Addgene has compiled various...
Showing: 81 - 100 of 201 results