...collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs....

...Publication RNA Targeting Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA...

...repository. Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more...

...tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...

... vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning...

...repository. Browse our optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....

... these viral vectors undergo quality control, including AAV titering by SYBR green qPCR , confirmation...

...expression of mouse Oct4, Klf4, c-Myc, and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental...

...full length protein is only made when the ncAA is included. For any and all questions you have while designing...

...article (listed below by PubMed ID). These factors include: Does a given gRNA sequence exactly match your ...

...plants Plant Plasmids for six gRNA module vectors, including three designed for dicots and three designed for...

..., Musicophilia includes case studies of relevant neurological atypicalities, including a patient with ...Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a book club in the office...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...protagonist, some clever magic, and a satisfying conclusion? In this series, there is always another secret...in 2011 and is still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director...explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous conversations...book-loving Addgenies. I’d never participated in a book club before, but I had a few reasons for launching this...

...up cell clumps Success in a flow cytometry experiment depends on a variety of factors including sample ...instead of vortexing. Include proper controls To ensure accurate data analysis, include a complete set of ... by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases, this...whole blood sample will contain a mix of cells including granulocytes, lymphocytes, and monocytes. Due ...different populations of immune cells express unique Clusters of Differentiation cell surface markers (CD) these... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...staining.  If you’re using antibodies for detection, include an unstained control with cells that went through...

...optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment...peptide. CRY2clust should prove a useful tool to further probe cellular function. The CRY2clust Plasmid...proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to ...CRY2, although some studies have shown that this clustering only happens under certain conditions. With...With its additional 9 amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization...reversible upon termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics...sensitivity to light, and better distribution of clusters without accumulation in subcellular compartments...

... antibodies, include controls where each primary is omitted but all secondaries included to uncover secondary... shifts indicative of tau phosphorylation. By including the positive control, you will be able to confirm... addition to experimental samples (?). B, The inclusion of biological replicates such as multiple mice...Replicates can be technical or biological and are included to improve the precision and accuracy and therefore...repeated measurements of the same sample and are included to assess variability in the experimental protocol...: Microscopy-based antibody experiments should include a primary antibody specificity control, secondary...antibody. The secondary antibody control should be included with each experimental run (Burry 2011). Autofluorescence...

...particular interest this fall:  The BioBuilderClub BioBuilderClub engages high school teams around the...runoff from winter icing.BioBuilderClub is the only informal synthetic biology club of its kind, running...classes, some pick and choose a few lessons to include each year; others use BioBuilder as an afterschool... work with one of five BioBuilder kits — which include lab materials and reagents such as droppers, centrifuge...biology.Launching with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group...completion, from pitch to prototype. Last year’s BioBuilderClub teams’ biological engineering designs and ideas... year from remote scientist mentors and the BioBuilderClub community. Registration is open through October...

...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...6). Many of the new administration’s actions, including taking down the climate change page on whitehouse.gov... consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that...with one unifying interest (the advancement and inclusion of science) they come from many different places...see and understand data themselves can come to conclusions themselves instead of depending on scientists...that ‘facts’ are subjective or alternative. Inclusion of data informing policy specifically related ...the most profound impact (15). Diversity and inclusion in science, technology, engineering and mathematics...

... the Chi-Bin Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping...promoters for cell types directly linked to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3...CyOFP1), some of the exciting properties of mCyRFP1 include a redshifted-emission spectrum compared to that...The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1 expression... was initially limited by off-target effects, including the conversion of other cytosines near the target...knockouts replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes... cassette via Cre recombination. Each knockout includes unique barcodes and universal priming sites to...

...you have many career options available to you including careers in academia, industry, non-profit, and... to maintaining a status in the United States including green cards that are well-tailored to meet the...specialized knowledge in a field of human endeavor including but not limited to biotechnology, chemistry, architecture...publications, and plan for future activities; including the interest of the greater scientific field, ...entities or individuals. A successful NIW case should include evidence such as proof of US government grants ...fact is best proved through objective criteria including awards, media attention, citations to your work... discuss your work. Participating as a judge (including as a peer reviewer for journals, acting as a journal...

... an array of post-translational modifications including protein folding, formation of disulfide bridges...for protein production and purification. These include mammalian, insect, bacterial, plant, yeast and ...summarize some of the more common expression systems including their advantages and caveats to keep in mind before...Some drawbacks of insect cell expression systems include that virus production can be quite time consuming...produced by bacteria become insoluble, forming inclusion bodies that are difficult to extract without harsh...requirements as mammalian cell expression systems, including the majority of complex post-translational modifications...been used to express a wide range of proteins including antibodies and pharmaceuticals, specifically interleukins...