DAP arrays for multiplex gene editing
Type
Blog Post
...expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ...
We narrowed to 145 results for: ENDOG
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Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post...Of note, recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This... -
Viral Vectors 101: Types of viruses
TypeBlog Post... that can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...purification as well as reversibly regulation of endogenous signaling activity in cells. Let’s shed some... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine, and are only activated... -
Hot Plasmids - February 2022
TypeBlog Post...levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...animals that are genetically altered to overexpress endogenous RNAi pathways. These mutations however can interfere... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...which restores eye color to fly lines where the endogenous white gene is knocked out. Flies with your integration... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...complex for the transcriptional activation of endogenous genes and it consists of three components: 1.... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...the IP landscape, using plant parts which are endogenous to the plant, off patent, or not patented, and... -
Zebrafish Plasmid Collection
TypeCollection...encoding fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0 - Jeff Mumm... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...Giovanni Pezzulo, and Joshua M. Finkelstein. "Endogenous bioelectric signaling networks: exploiting voltage... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...proteins are most widely used tags because they emit endogenous fluorescence that allow easy detection with the... -
Human Germline Editing Using CRISPR
TypeBlog Post...than using term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post... prevent aberrant binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...protocols that use antibody fragments to block the endogenous immunoglobulins and reduce the background staining...