We narrowed to 142 results for: ENDOG

1. Viral Vectors 101: Viral Vector Elements

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   ...Of note, recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This...

2. Viral Vectors 101: Types of viruses

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   ... that can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning...

3. Hot Plasmids - February 2022

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   ...levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells...

4. GPCRs: How Do They Work and How Do We Study Them?

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   ...chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine, and are only activated...

5. Luminescent Imaging with Nano-lanterns

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   ...also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs...

6. Top Requested Lentivirus and AAV of 2016

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   ...recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi...

7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...animals that are genetically altered to overexpress endogenous RNAi pathways. These mutations however can interfere...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...purification as well as reversibly regulation of endogenous signaling activity in cells.  Let’s shed some...

9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...which restores eye color to fly lines where the endogenous white gene is knocked out. Flies with your integration...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, ...

11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...complex for the transcriptional activation of endogenous genes and it consists of three components: 1....

12. 3 Challenges in Plant Synthetic Biology

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    ...the IP landscape, using plant parts which are endogenous to the plant, off patent, or not patented, and...

13. CRISPR Plasmids - Cascade-Cas3

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    ..., and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class ...

14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...proteins are most widely used tags because they emit endogenous fluorescence that allow easy detection with the...

15. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...Giovanni Pezzulo, and Joshua M. Finkelstein. "Endogenous bioelectric signaling networks: exploiting voltage...

16. Human Germline Editing Using CRISPR

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    ...than using term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous...

17. Viral Vectors 101: Calcium Sensors

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    ... prevent aberrant binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your...

18. Antibodies 101: Choosing the Right Isotype

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    ...protocols that use antibody fragments to block the endogenous immunoglobulins and reduce the background staining...

19. Hot Plasmids and Viral Preps - March 2021

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    ...your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had...