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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 103 results for: EXO

Showing: 81 - 100 of 103 results

  4. Distribution to Industry

    Type
    Collection
    ...Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these...mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening...

  5. Antibodies 101: Validation

    Type
    Blog Post
    ... causes a large deletion that removes the entire exon that codes for the epitope recognized by your antibody...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...Pancreatic Exocrine Cells Beta-cells Adenoviral Mouse In vivo reprogramming of adult pancreatic exocrine cells...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ... expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids...

  13. Rett Syndrome

    Type
    Collection
    ...Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...direction of these ORF fragments (which have become exons) determines the logical expression requirements ...

  17. Validated gRNA Sequences

    Type
    Collection
    ...knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments...

  18. Cre-lox system

    Type
    Collection
    ...: These constructs require the addition of an exogenous ligand (e.g. tamoxifen) to activate Cre. One advantage...

  19. Cloning

    Type
    Guide
    ...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes... thus does not need restriction enzymes or an exogenous ligase providing an incredibly quick and easy ...together in one isothermal reaction. First the 5’ exonuclease chews back the 5’ ends of your DNA fragments ...polymerase then closes the gap created by the 5’ exonuclease and finally ligase seals the nicks in the DNA...potential to be completely chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson... DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates “chewed-back” overhangs of 10...

  20. Plan Your Experiment

    Type
    Guide
    ...Cas9 nickase) Single (or dual) gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes...gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances that the targeted region...removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since frameshift... Alternatively, gRNAs can be designed to target exons that code for known essential protein domains. The...
Showing: 81 - 100 of 103 results