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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 103 results for: EXO

Showing: 81 - 100 of 103 results

  3. Antibodies 101: Validation

    Type
    Blog Post
    ... causes a large deletion that removes the entire exon that codes for the epitope recognized by your antibody...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9...Mulvihill 110486 Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC... 110488 pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc ...EIF2AK2 SV40 VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD..._400 FUS Halo ALS Michael Ward 162274 HTT delta-exon1 HTT Flag CMV, P10 Huntington's Cheryl Arrowsmith...

  6. Distribution to Industry

    Type
    Collection
    ...Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these...mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...Pancreatic Exocrine Cells Beta-cells Adenoviral Mouse In vivo reprogramming of adult pancreatic exocrine cells...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ... expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids...

  14. Rett Syndrome

    Type
    Collection
    ...Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...direction of these ORF fragments (which have become exons) determines the logical expression requirements ...

  18. Validated gRNA Sequences

    Type
    Collection
    ...knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments...

  19. Cloning

    Type
    Guide
    ...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes... thus does not need restriction enzymes or an exogenous ligase providing an incredibly quick and easy ...together in one isothermal reaction. First the 5’ exonuclease chews back the 5’ ends of your DNA fragments ...polymerase then closes the gap created by the 5’ exonuclease and finally ligase seals the nicks in the DNA...potential to be completely chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson... DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates “chewed-back” overhangs of 10...

  20. Plan Your Experiment

    Type
    Guide
    ...regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces ... expression of a truncated protein. Targeting an exon coding for an essential protein domain could abolish...
Showing: 81 - 100 of 103 results