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We narrowed to 100 results for: EXO

Showing: 81 - 100 of 100 results
  1. Antibodies 101: Validation

    Type
    Blog Post
    ... causes a large deletion that removes the entire exon that codes for the epitope recognized by your antibody...
  2. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...or Cas9 domains PE7 — addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...
  3. Zebrafish Plasmid Collection

    Type
    Collection
    ... expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids...
  4. Molecular Cloning Techniques

    Type
    Guide
    ...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity... thus does not need restriction enzymes or an exogenous ligase, providing an incredibly quick and easy...of three common molecular biology enzymes: 5' exonuclease, polymerase, and ligase. In Gibson assembly, ...together in one isothermal reaction. First, the 5’ exonuclease chews back the 5’ ends of your DNA fragments,...polymerase then closes the gap created by the 5’ exonuclease. Finally, ligase seals the nicks in the DNA to...potential to be completely degraded by the 5' exonuclease. Read more in our Gibson Assembly blog post ....T4 polymerase will continue to function as an exonuclease until a base is exposed on the single-strand ...
  5. Rett Syndrome

    Type
    Collection
    ...Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion...
  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...direction of these ORF fragments (which have become exons) determines the logical expression requirements ...
  7. Plan Your Experiment

    Type
    Guide
    ...regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces ... expression of a truncated protein. Targeting an exon coding for an essential protein domain could abolish...
  8. CRISPR Guide

    Type
    Collection
    ...libraries often target 5′ constitutively expressed exons, while activation and repression libraries target...
  9. CRISPR Guide

    Type
    Guide
    ...libraries often target 5′ constitutively expressed exons, while activation and repression libraries target...
Showing: 81 - 100 of 100 results