We narrowed to 342 results for: GAI
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TypeBlog Post...applications. But some techniques require you to select against the presence of a transgene instead, which can’...are currently used in these situations to select against CRISPR positive plants, but screening these can...
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Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...as you will likely need a functional prototype to gain any real traction. This can carry a heavy price...company advancement. Be relentless The world is against you. As I mentioned, you start at a disadvantage... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...plasmids in the library. Analyzing the reads is, again, seemingly straightforward – you need to count how...calculating the area under the curve (AUC), one can gain a sense of the uniformity of the library distribution... -
Celebrate National Book Month with Addgene
TypeBlog Post...lost for an age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. ...reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...school, and it’s oddly fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen... universities. However, much of the information gained from that research remains inaccessible, behind... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...experiment by simply running it unchanged over and over again, and the same goes for your workflow. Next time ...mistake, instead of silently vowing never to do that again (I’ve been there!), think of ways you could change... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... to generate two more OptoNBs, one against mCherry and one against GFP. Insertion of AsLOV2 into loop ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post... interactive plasmid map powered by SnapGene and gain access to additional tooltip information and sequence...Additionally, align a custom nucleotide sequence against a given sequence using BLAST2. See this blog post... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post... gather more feedback, and start the cycle over again to refine the solution, or to come up with a new...your network to your advantage As the profession gains more traction in the sciences, I believe that more... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post... with ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around...really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly... freezes and then crashes. You’re muted on Zoom again. These things don’t just happen to you or me (though... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...for every assay. Antibodies that are generated against a linear antigen tend to work very well for applications...compare the staining patterns of multiple antibodies against the same target; tagged protein strategies that... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold... and anatomical techniques, her research aims to gain a deeper understanding of the physiology and pathology... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...stated, ccdB makes cloning easier by selecting against vectors that did not take up your insert. But exactly...antitoxin protein (CcdA) that protects the cell against the toxic CcdB. Cells that lose ccdA through the... -
The Future of Research Symposium Boston 2015
TypeBlog Post...PhDs Later this week, postdocs and students will again gather in Boston - this time with the aim of finding...University, has recently published an article arguing against reducing the number of graduate students accepted... -
A Look at Addgene's QC Process
TypeBlog Post...called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided. If no...plasmid, and the full QC process is started over again after receiving the replacement plasmid at Addgene... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...the run completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of...failed” and removed from the plate to be prepped again. These holes are then filled with other samples ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...media or journalism course. Courses can help you gain work experience and build your network, but don’...Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...collection of CRISPR tools has grown steadily, gaining approximately 2,000 plasmids/year from 2017 to ...most popular with our requestors. However, we can again see quick adaptation and steady use of all types...