We narrowed to 410 results for: HRE

...content_id":null,"href":"https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org... plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing...

...have been a staple in molecular biology for over three decades. Widely used across various research applications...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically processed... entry into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

...environment and the cytoskeleton. Most integrins have three conformational states: bent-closed, extended-closed...processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...individually and in combination, showing that among the three αV integrins expressed on the BJ-5ta fibroblast ...

...proteins (5). Optimization of this system defined three components required for translation (Figure 1): ...translation of the pSer-incorporated polypeptide. These three components were shown to effectively incorporate...that wasn’t originally a serine (e.g. mutating a threonine residue to a phosphoserine) to control for effects...

...’t been used to treat disease postnatally. Now, three papers published concurrently in Science have shown...dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin expression via Western..., and Niemann-Pick disease type C. Although the three DMD studies referenced here represent a great step...

...a set duration of only three months and made a good job to list on my CV. Three months was a perfect duration...needed to be resourceful and adapt. By the end of my three months, I fully understood why parasitology roles...

...packaging viral vectors.   Let’s take a closer look at three different transposon systems adapted for use as ... the crRNA, 3) a transposase complex made up by three transposase proteins (TnsA, TnsB, TnsC), and 4) ...its transposition protein, to move DNA. There are three popular systems for use in the lab (Sleeping Beauty...

...loosely organized tissues that express markers of all three germ layers (ectoderm, mesoderm, endoderm). Measuring...shows the ability of the iPSC to contribute to all three germ layers, and the ability of theses chimaeras...looking for tumors that contains cells from all three germ layers. Choosing a reprogramming method Most...

...much debate, we have finally narrowed it down to three choices.   Fig. 1: Lin, Abi, or Blottie?...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

...this process easy and fun every day.  With these three main values we set out to radically redesign our...content, and made a commitment to reinforcing the three values with each element. We also set out to make...

...databases and resources, check out our growing Twitter thread. We’re glad to see the scientific community work...list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   ...

... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...selectivity for norepinephrine over dopamine. All three sensors, however, did display some reaction to high...

...max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog. These were a miR30...surprises. Originally we thought during the first three weeks that we had made a mistake by not having the...

...Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, I had put on...

...powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR...system (4). Makarova et al. grouped the systems into three distinct types and subtypes using polythetic classification...

... used the Cre-loxP system to express one of the three fluorescent protein (XFP) genes in a transgene. ...will result in a combinatorial expression of these three genes with different copy numbers, producing dozens...

... μg/mL to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and...use of XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to ...

... the hairpin combination. A gRNA containing all three hairpins produces a white color. While CRISPRainbow... the loci of interest, the team could visualize three loci and measure inter-locus distances. They tested...

... break, we determined that Cas9 held tightly to three of the four strands of the cleaved duplex, while... drive sequence replacement in cells. Targeting three dCas9 molecules to a precisely spaced region allowed...

...present at certain times during the day? We asked three lab heads how they go about selecting new hires....How do I convince the PI to choose me?” We asked three leading professors how they hire scientists for ...