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We narrowed to 407 results for: HRE

Showing: 81 - 100 of 407 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...have been a staple in molecular biology for over three decades. Widely used across various research applications...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically processed... entry into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...proteins (5). Optimization of this system defined three components required for translation (Figure 1): ...translation of the pSer-incorporated polypeptide. These three components were shown to effectively incorporate...that wasn’t originally a serine (e.g. mutating a threonine residue to a phosphoserine) to control for effects...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...environment and the cytoskeleton. Most integrins have three conformational states: bent-closed, extended-closed...processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...individually and in combination, showing that among the three αV integrins expressed on the BJ-5ta fibroblast ...

  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...vivo (Allocca et al., 2008). Shortly after though, three groups independently tried reproducing these results... Today’s blog post will give brief overviews of three split vector strategies plus one additional split...mini-dystrophin gene to a mouse model of muscular dystrophy. Three different splice site donor and acceptor sequences...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...’t been used to treat disease postnatally. Now, three papers published concurrently in Science have shown...dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin expression via Western..., and Niemann-Pick disease type C. Although the three DMD studies referenced here represent a great step...

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    ...a set duration of only three months and made a good job to list on my CV. Three months was a perfect duration...needed to be resourceful and adapt. By the end of my three months, I fully understood why parasitology roles...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...packaging viral vectors.   Let’s take a closer look at three different transposon systems adapted for use as ... the crRNA, 3) a transposase complex made up by three transposase proteins (TnsA, TnsB, TnsC), and 4) ...its transposition protein, to move DNA. There are three popular systems for use in the lab (Sleeping Beauty...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...loosely organized tissues that express markers of all three germ layers (ectoderm, mesoderm, endoderm). Measuring...shows the ability of the iPSC to contribute to all three germ layers, and the ability of theses chimaeras...looking for tumors that contains cells from all three germ layers. Choosing a reprogramming method Most...

  9. Our New Antibody Mascot is....

    Type
    Blog Post
    ...much debate, we have finally narrowed it down to three choices.   Fig. 1: Lin, Abi, or Blottie?...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...selectivity for norepinephrine over dopamine. All three sensors, however, did display some reaction to high...

  14. An Addgene Summer Internship

    Type
    Blog Post
    ...max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog. These were a miR30...surprises. Originally we thought during the first three weeks that we had made a mistake by not having the...

  15. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR...system (4). Makarova et al. grouped the systems into three distinct types and subtypes using polythetic classification...

  16. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... used the Cre-loxP system to express one of the three fluorescent protein (XFP) genes in a transgene. ...will result in a combinatorial expression of these three genes with different copy numbers, producing dozens...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... μg/mL to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and...use of XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to ...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... the hairpin combination. A gRNA containing all three hairpins produces a white color. While CRISPRainbow... the loci of interest, the team could visualize three loci and measure inter-locus distances. They tested...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... break, we determined that Cas9 held tightly to three of the four strands of the cleaved duplex, while... drive sequence replacement in cells. Targeting three dCas9 molecules to a precisely spaced region allowed...

  20. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...present at certain times during the day? We asked three lab heads how they go about selecting new hires....How do I convince the PI to choose me?” We asked three leading professors how they hire scientists for ...
Showing: 81 - 100 of 407 results