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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10 results for: HRE

Showing: 1 - 10 of 10 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...: 450 µL PBS only. Pipette 50 µL into the first three wells of each row, using a different standard solution... 1 min . Repeat steps 8–10 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 18–20 twice for a total of three washes. After the final wash, aspirate the wash ...empirically determined. We suggest starting with three concentrations between 1–10 µg/mL. Cap and invert...1 min . Repeat steps 30–32 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 40–42 twice for a total of three washes. After the final wash, aspirate the wash ...

  2. Gibson Assembly Protocol

    Type
    Protocol
    ...single isothermal reaction. With the activities of three different enzymes, the product of a Gibson Assembly...master mix (isothermal reaction mix) consists of three different enzymes within a single buffer mixture...

  4. Weighing Reagents Protocol

    Type
    Protocol
    ... need 300 g of sucrose, you can weigh out 100 g three times, placing the sucrose in your container after...

  5. Lentivirus Production

    Type
    Protocol
    ...°C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into OptiPro SFM: Reagent ...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...series is prepared for each AAV sample, and the three final dilutions are assayed. The samples that are...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    .... Using the total number of base pairs from all three plasmids, we can determine the total μg/bp we need...

  10. CRISPR Library Amplification

    Type
    Protocol
    ... 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders...
Showing: 1 - 10 of 10 results