...: 450 µL PBS only. Pipette 50 µL into the first three wells of each row, using a different standard solution... 1 min . Repeat steps 8–10 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 18–20 twice for a total of three washes. After the final wash, aspirate the wash ...empirically determined. We suggest starting with three concentrations between 1–10 µg/mL. Cap and invert...1 min . Repeat steps 30–32 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 40–42 twice for a total of three washes. After the final wash, aspirate the wash ...

...single isothermal reaction. With the activities of three different enzymes, the product of a Gibson Assembly...master mix (isothermal reaction mix) consists of three different enzymes within a single buffer mixture...

...working with low-risk microbes posing little to no threat of infection or disease in healthy humans. Many...

... need 300 g of sucrose, you can weigh out 100 g three times, placing the sucrose in your container after...

...°C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into OptiPro SFM: Reagent ...

...to the diluted DNA. Cap the tube and vortex with three 1 s pulses. Incubate for 3 min at room temperature...

...NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009 PageRuler Plus Prestained...

...series is prepared for each AAV sample, and the three final dilutions are assayed. The samples that are...

.... Using the total number of base pairs from all three plasmids, we can determine the total μg/bp we need...

... 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders...