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We narrowed to 10 results for: HRE

Showing: 1 - 10 of 10 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...In order to produce lentiviral vectors, you need three (or four, for third generation) plasmids: Transfer...VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the necessary viral genes...replication incompetent by using four plasmids instead of three and eliminates the requirement for Tat. Always includes...lentiviral production. All lentiviral plasmids (three or four) are transfected into HEK293T cells using...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...’s Molecular Biology Reference . Production The three production plasmids (transfer, packaging, and helper...Rep52, and Rep40. Cap in trans The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...order to produce gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains..., similar to lentiviral packaging methods . The three plasmids described above (envelope, packaging, and...for viral vector production are separated into three different plasmids containing the transfer, envelope...

  4. Molecular Biology Reference

    Type
    Guide
    ...V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code Codons (RNA) Alanine... CCG Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG ...GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...

  5. Promoters

    Type
    Guide
    ...insulators, and silencers. Promoter Regions There are three main portions that make up a promoter: core promoter... initiate the transcription of genes. There are three types of RNA polymerases that all transcribe different...

  6. Modular Cloning Guide

    Type
    Guide
    ...gene-editing tools, and more. The MoClo system consists of three sets of cloning vectors (Level 0, 1, and 2) which...Rossignol 64 plasmids to assemble and integrate up to three transcription units in the oleaginous yeast Y. lipolytica...

  7. Antibody Guide

    Type
    Guide
    ...Production Antibodies are typically produced in one of three different methods: in animals, in cultured immune...secondaries, are usually much easier to select. There are three main considerations. What is the source of your ...

  8. Chemogenetics Guide

    Type
    Guide
    ...intracellular calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: ...

  9. Plan Your Experiment

    Type
    Guide
    ...than 10 bp away. For more information, read our three tips to improve HDR efficiency . Remember that HDR...

  10. CRISPR Guide

    Type
    Guide
    ...target region, effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions...
Showing: 1 - 10 of 10 results