Skip to main content
Addgene
Showing: 1 - 20 of 398 results

  6. Cre-lox system

    Type
    Collection
    ...CMV AAV Nestler 68552 HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570...will be rearranged. The schematic below shows the three types of rearrangements: inversion, deletion, and...Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre Cre EF-1 alpha AAV Engel...

  7. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...: 450 µL PBS only. Pipette 50 µL into the first three wells of each row, using a different standard solution... 1 min . Repeat steps 8–10 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 18–20 twice for a total of three washes. After the final wash, aspirate the wash ...empirically determined. We suggest starting with three concentrations between 1–10 µg/mL. Cap and invert...1 min . Repeat steps 30–32 twice for a total of three washes. After the final wash, aspirate the wash ...1 min . Repeat steps 40–42 twice for a total of three washes. After the final wash, aspirate the wash ...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent... Feb 13;347(6223):755-60. Loren Looger , Eric Schreiter Calcium Intersectional viral expression of sRGECO...chemigenetic indicators. bioRxiv 2020.01.08.898783 Eric Schreiter Calcium WHaloCaMP1 chemigenetic calcium indicator...near-infrared light. bioRxiv 2023.07.18.549527 Eric Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium...Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with a Genetically...dendrites (ArcLight) Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent...Vincent Pieribone Voltage ArcLight-ST to detect subthreshold membrane potential changes Simultaneous two-...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV.hSyn.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-1-PV3844 100855-AAV1 pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2...pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...F391W-WPRE-SV40 Biosensor Eric Schreiter AV-5-52925 52925-AAV5 pZac2.1 gfaABC1D-cyto-GCaMP6f Biosensor ...pAAV.hSyn.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-5-PV4090 100831-AAV5 pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40...pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-9-PV2723 98929-AAV9 pAAV.hSyn.iGluSnFr.WPRE....pAAV.hSyn.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-9-PV3849 100854-AAV9 pAAV.Syn.NES-jRGECO1a....

  10. Biosensor AAV Preps

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor: HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP...pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors 111066 pAAV-hSyn-dLight1.1 Syn...pAAV-hsyn-flex-Voltron-ST Syn Voltron-ST none Cre dependent 1 Schreiter 172907 pGP-AAV-syn-FLEX-Volton2-ST-WPRE Syn Voltron2...

  11. Ras Pathway

    Type
    Collection
    ...antagonist STK STK3 STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase...binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene...oncogenes and their downstream targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given site, the... activation of endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64...to express SpCas9, and another to express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014...modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma...

  13. TALEN Engineering

    Type
    Collection
    ...plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays...Reyon and Tsai et al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat ...architectural framework and the DNAs produced by all three methods are identical in sequence. TAL effector ...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...KIR3DL1 killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 CD158E1, KIR, ...KIR3DL2 killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2 CD158K, MGC125321...KIR3DL3 killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 3 CD158Z, KIR3DL7...morphogenetic protein receptor, type II (serine/threonine kinase) BMPR-II, BMPR3, BMR2, BRK-3, FLJ41585,...releasing hormone receptor GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth... 1, group D, member 1 EAR1, THRA1, THRAL, ear-1, hRev NR1D2 nuclear receptor subfamily 1, group D, member...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...c-Myc, Nanog, Lin28, NR5A2, and microRNA 302/367 in three different combinations of reprogramming factors ...human Oct4, Sox2, Myc, Klf4, BCL2L1, and EBNA1 from three separate plasmids A Facile Method to Establish Human... iXENs Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts...

  16. TALEN Expression Vectors

    Type
    Collection
    ...can be found on the TALengineering website . All three of these methods yield TAL effector repeat arrays...details of the assembly are different among the three methods, the final products are the same). All expression...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...six gRNA module vectors, including three designed for dicots and three designed for monocots. Using these...genome editing and transcriptional regulation Plant Three types of plasmids available for use in this Golden...

  18. Gibson Assembly Protocol

    Type
    Protocol
    ...single isothermal reaction. With the activities of three different enzymes, the product of a Gibson Assembly...master mix (isothermal reaction mix) consists of three different enzymes within a single buffer mixture...

  19. mTOR Pathway

    Type
    Collection
    ... growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1...LST8 homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin...

  20. Microbiology Resources

    Type
    Collection
    ... Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs...tool kit : Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica...
Showing: 1 - 20 of 398 results