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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 185 results for: INV-E

Showing: 81 - 100 of 185 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab... methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...complex constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

  3. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells...efficiency of the method. One observation was an inverse relationship between deletion size and frequency...outcome to deletions following paired DSBs is inversions [3]. These rearranged segments may also be informative...nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with...CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, ...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...recombination persists even in recombinase deficient E. coli and under conditions used to minimize recombination...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...gate cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...well as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the... MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains...domains, but these domains often do not express well in E. coli without their partner regulatory domains. The...library and used this library to screen for genes involved in West Nile Virus (WNV) induced cell death. The...for their gene of interest. Although the parts involved in altering gene expression are relatively well...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, ...results in effective gene delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ....org/10.1126/science.1231143 Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...

  9. Reflections and Looking Ahead

    Type
    Blog Post
    ..., A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman... plasmids — as DNA or bacterial stabs — ‌and reinventing our search experience so you can find exactly...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...

  14. What the HEK?

    Type
    Blog Post
    ...2002) (8):869-71. DOI: 10.1096/fj.01-0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce...interests include receptor biology, ion channels, or involve human protein preparations with PTMs. Why the HEK...

  18. How Addgene Creates Content

    Type
    Blog Post
    ...website, social media, videos, newsletters, guides, and e-books – comes into play. Our goal is to provide informative...post, we invite you behind the scenes in our content writing process, and to even get involved yourself...standards. It’s always a work in progress! Get involved A lot of our content already comes from you and...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and tell us...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq...lab targets human genes known or suspected to be involved in DNA damage response and DNA repair.  A new ...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...10.1039/C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., ...animals being monitored were physically hooked up to invasive, cumbersome systems during experiments – neither...
Showing: 81 - 100 of 185 results