...of these orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for ...color is specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence...in combination with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce ...

...are a non-renewable reagent - Their production requires animal sacrifice and because they are an animal...secondary Less sensitive than HRP-RANbody staining Requires secondary antibody staining HA Tagged GFP, RFP...secondary   Less sensitive than HRP-RANbody staining Requires secondary antibody staining GFP, RFP, H2A2B ...

..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...

...handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less involved setup when working...Created with BioRender.com. The in vitro option requires a bit less work for the first two steps, as no...

... Park.  In addition to Taq DNA polymerase, PCR requires free nucleotides (dNTPs), template DNA to amplify...measuring a starting DNA concentration using PCR. qPCR requires the addition of a probe based fluorescent dye ...transcription-qPCR (RT-qPCR). The first step of this process requires RNA to be converted to cDNA using reverse transcription...

... Digital or print distribution.  Static. Often requires both artistic and scientific training. Cartoons...media type to another is cognitively demanding. Requires multiple areas of expertise.  Social Media ...scicomm. Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls" ...

...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...be accessed. Adding to this burden, the policy requires that data should be available for anonymous request...data authors. In some types of research, raw data requires huge amounts of storage. For example, mouse behavioral...

...incorporate new information into DNA. This also requires an exogenous DNA template. HDR, however, is a ...lightly! Indeed, truly achieving the gold standard requires not one but two rounds of single cell cloning ...limited to single nucleotide transitions but still requires a nearby PAM, although these are still early days...

...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...or protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and...exogenous protein, successful induction of RNAi only requires the presence of dsRNA. In plants, CRISPR technologies...RNAi-based investigation. Creation of the RNAi trigger requires a few initial considerations, such as the starting...

...and no longer requires drug selection.  One drawback to this approach is that it requires the generation...occur in a test tube. The donor DNA substrate only requires ~50 nucleotides of homology to the target site...

...disease” often requires understanding the disease genetics and the “treatment” usually requires drugs. While...

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...  To start, the average health science startup requires a great deal more capital to get off the ground...

... The entire protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. ...Dickinson et al. have developed a modular system that requires very little hands-on labor and could facilitate...

...provides a truly random digestion, but is harsher and requires more optimization. Enzymatic digestion, while ...https://www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody...

...though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate and integrate into...target different tissue or cell types. AAV also requires some back up from adenovirus genes – E4, E2a, ...

...capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in order to...Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture, so bacteria...

...like-minded people, I quickly realize that a company requires more than just good data to become a product. ...and succeeding. Take home Starting a company requires dedication, passion, and around-the-clock work...

... paper for publication in a scientific journal requires several considerations. First, it is important.... If the reviewers suggest that the manuscript requires revisions, the authors can publish a new version...